Anti-CBP80 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q09161

Core Product Specifications and Parameters

Biological Background: CBP80 Function and Localization

• Nuclear cap-binding protein subunit 1 (NCBP1), also known as CBP80 or 80 kDa nuclear cap-binding protein, is the large subunit of the cap-binding complex (CBC).
• The CBC binds cotranscriptionally to the 5' cap of pre-mRNAs and participates in multiple RNA processing events, including pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay (NMD), microRNA (miRNA) biogenesis, and mRNA export.
• CBP80 does not directly bind the capped RNA; instead, it stabilizes the cap-binding loop of NCBP2/CBP20, locking the CBC into a high-affinity cap-binding state. Additionally, CBP80 can associate with NCBP3 to form an alternative CBC that specifically mediates mRNA export, particularly during cellular stress.
• The CBC complex is essential for the pioneer round of translation, which is tightly linked to NMD. It enhances NMD through interaction with UPF1 and supports both exon-junction complex (EJC)-dependent and failsafe NMD pathways.
• CBP80 is involved in miRNA-mediated gene silencing and pri-miRNA processing through its interaction with SRRT/ARS2.
• The protein localizes to both the nucleus and cytoplasm, consistent with its roles in nuclear mRNA processing and export as well as cytoplasmic translation surveillance.
• Required for cell growth and viability. Related references: PMID:26382858
• Post-translationally modified by acetylation, phosphorylation, and ISGylation (isopeptide bond).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human CBP80; homology with mouse in this region should be considered for cross-reactivity.
• For Western blot, the predicted molecular weight is approximately 92 kDa; validate band size in your sample system.
• For IHC-P, consider antigen retrieval conditions and appropriate controls.
• As a polyclonal antibody, evaluate lot-to-lot consistency for critical applications.
• Validated for human and mouse; for other species, in silico epitope conservation may be checked.

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CamelBio streamlines your IVD raw material procurement by offering validated antibodies and reagents for mRNA processing and nuclear transport research. From this CBP80 polyclonal antibody to custom antibody pairs and bulk ancillary reagents, we bridge the gap between concept and clinic. Contact us to source rare targets and scale your diagnostic development.