Anti-CBLL1 Rabbit Polyclonal Antibody for WB and ELISA - Q75N03

Core Product Specifications and Parameters

Biological Background: CBLL1 Function and Localization

• CBLL1, also known as Hakai, RNF188, or c-Cbl-like protein 1, is an E3 ubiquitin-protein ligase that mediates ubiquitination of tyrosine-phosphorylated Src substrates, including CDH1, CTTN, and DOK1.
• As a core component of the WMM complex, CBLL1 contributes to N6-methyladenosine (m6A) methylation of RNAs, influencing mRNA splicing and processing efficiency. Related references: PMID:29507755
• The protein localizes to the nucleus, notably in nuclear speckles and nucleoplasm, but is also found in the cytoplasm.
• CBLL1 targets CDH1 (E-cadherin) for ubiquitination, leading to its endocytosis and degradation.
• Structural features include a RING finger domain and zinc-finger motifs, consistent with its RING-type E3 ubiquitin transferase activity.
• Post-translational modifications such as phosphorylation have been reported, and it is classified as a developmental protein.
• Alternative splicing produces multiple isoforms, and it has been identified in large-scale proteomics analyses as part of the reference proteome.

Experimental Guidance and Technical Tips

• The immunogen comprises the N-terminal region (amino acids 1-240), making the antibody well-suited for detecting full-length CBLL1 in Western blot experiments.
• For ELISA applications, optimization of coating and detection conditions is recommended based on the target antigen’s characteristics.
• Given the reported cross-reactivity with human and mouse, consider using lysates from species-appropriate cell lines (e.g., HeLa, HEK293, NIH/3T3) for specificity validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories streamlined access to IVD raw materials, including validated antibodies and ancillary reagents. For studies on ubiquitination or m6A methylation pathways, we can assist in sourcing high-quality CBLL1 antibodies and complementary tools to accelerate your assay development. Our comprehensive sourcing services support your workflow from concept to clinic.