Anti-CBLC Rabbit Polyclonal Antibody for WB, ELISA - Q9ULV8

Core Product Specifications and Parameters

Biological Background: CBLC Function and Localization

• CBLC (CBL-3, RNF57) is a RING finger E3 ubiquitin-protein ligase that mediates the transfer of ubiquitin from E2 enzymes to target substrates, promoting proteasomal degradation.
• It is functionally coupled with the E2 ubiquitin-conjugating enzymes UB2D1, UB2D2, and UB2D3, highlighting its role in the ubiquitin conjugation pathway.
• CBLC regulates epidermal growth factor receptor (EGFR) signaling by ubiquitinating EGFR upon EGF stimulation; isoform 1, but not isoform 2, inhibits EGF-stimulated MAPK1 activation.
• The protein promotes ubiquitination of SRC phosphorylated at Tyr-419 and, in collaboration with CD2AP, modulates the rate of RET degradation, thereby acting as a regulatory checkpoint for RET signaling.
• CBLC is ubiquitously expressed across tissues, consistent with its fundamental role in cellular signaling homeostasis.
• Structural features include calcium-binding sites, zinc-finger domains, and SH3-binding motifs; it is subject to alternative splicing and phosphorylation, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• For Western blot, consider using whole cell lysates from human or mouse cell lines; validate antibody specificity with appropriate controls (e.g., siRNA knockdown or overexpression).
• The immunogen covers amino acids 245–474, a region that may contain functional domains; verify the detected band near the predicted molecular weight of 52 kDa.
• In ELISA, the immunogen sequence can serve as a coating antigen for titer determination; optimize coating conditions and secondary detection reagents.
• Given its role in ubiquitination, consider treating cells with proteasome inhibitors (e.g., MG132) to stabilize ubiquitinated substrates prior to detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in supplying IVD raw materials to diagnostic manufacturers and research institutes, offering a streamlined path from concept to clinic. For ubiquitination and signal transduction targets like CBLC, we provide validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and sourcing services for rare or custom raw materials. Let CamelBio be your reliable partner in building robust diagnostic assays.