Anti-CBL Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P22681

Core Product Specifications and Parameters

Biological Background: CBL Function and Localization

• CBL (Casitas B-lineage lymphoma proto-oncogene, also known as CBL2, RNF55, C-CBL) is an E3 ubiquitin-protein ligase that acts as a negative regulator of many signaling pathways by mediating ubiquitination of cell surface receptors. Related references: PMID:10514377, PMID:11896602, PMID:14661060
• It accepts ubiquitin from E2 enzymes and transfers it to substrates, promoting proteasomal degradation; recognized substrates include activated receptor tyrosine kinases such as KIT, FLT1, FGFR, PDGFRA, EGFR, and SRC family kinases. Related references: PMID:15190072, PMID:18374639, PMID:21596750, PMID:11896602
• CBL is involved in LAG3-mediated inhibition of TCR signaling by catalyzing Lys-63-linked ubiquitination of LAG3, and in removal of NECTIN1 from cell surface upon herpes simplex virus infection. Related references: PMID:40101708, PMID:28381567
• Essential for osteoclastic bone resorption, the Tyr-731 phosphorylated form of CBL recruits and activates PI3K at the cell membrane, a critical step for osteoclast function. Related references: PMID:14739300
• Subcellularly, CBL localizes to the cytoplasm, cell membrane, cilium, and Golgi apparatus.
• The protein is a zinc-binding phosphoprotein with a RING finger domain, classified as a proto-oncogene with disease-associated variants.

Experimental Guidance and Technical Tips

• For Western blotting, the expected band is ~100 kDa; consider using a suitable positive control lysate (e.g., hematopoietic or cancer cell lines) and validate the observed band with relevant blocking peptides or knockdown controls.
• For immunohistochemistry (IHC-P), antigen retrieval may be necessary; validate staining specificity by including isotype controls and testing in known positive and negative tissues.
• Due to the polyclonal nature, lot-to-lot consistency should be confirmed for critical quantitative assays.
• Cross-reactivity with mouse and rat allows potential use in rodent models; however, it is advisable to validate performance in the specific sample system beforehand.
• For ELISA, optimize coating conditions and consider pairing with a suitable detection antibody for sandwich assay development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio facilitates direct access to high-quality IVD raw materials for diagnostic developers and research laboratories. This anti-CBL polyclonal antibody supports investigations into ubiquitin-mediated signaling and oncogenic pathways. Our supply capabilities include validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development pipeline from concept to clinic.