Anti-CBL Rabbit Monoclonal Antibody for WB, IHC-P, IP, ELISA - P22681

Core Product Specifications and Parameters

Biological Background: CBL Function and Localization

• CBL encodes the E3 ubiquitin-protein ligase CBL, also known as Casitas B-lineage lymphoma proto-oncogene, c-Cbl, or RING finger protein 55. It is a proto-oncogene and member of the CBL family of RING-type E3 ubiquitin transferases.
• Functions as a negative regulator of numerous signaling pathways by catalyzing the ubiquitination and subsequent proteasomal degradation of activated cell surface receptors, including EGFR, KIT, PDGFRA, and PDGFRB. Related references: PMID:10514377, PMID:11896602, PMID:14661060, PMID:15190072, PMID:21596750.
• Recognizes and mediates ubiquitination of membrane-bound SRC family kinases, such as HCK and SRC, leading to their degradation. Related references: PMID:11896602.
• Plays an essential role in osteoblast differentiation and apoptosis, and is critical for osteoclastic bone resorption via a signaling pathway involving phosphatidylinositol 3-kinase activation. Related references: PMID:14739300, PMID:15190072, PMID:18374639.
• Participates in signal transduction in hematopoietic cells; also involved in LAG3-mediated T-cell regulation and HSV-1-induced NECTIN1 downregulation. Related references: PMID:28381567, PMID:40101708.
• Subcellular localization includes cytoplasm, cell membrane, cell projections (cilia), and Golgi apparatus. Post-translational modifications include phosphorylation (e.g., at Tyr-731) and autoubiquitination, which modulate its activity and interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 850–906 near the C-terminus of human CBL; consider this region when designing detection strategies for full-length vs. truncated isoforms.
• Recommended for Western blotting (WB) due to the high molecular weight of CBL (~100 kDa); ensure adequate transfer conditions for large proteins.
• For immunohistochemistry (IHC-P), validate with appropriate positive and negative control tissues, considering CBL’s cytoplasmic and membrane localization.
• In immunoprecipitation (IP), confirm efficient capture of the target and co-immunoprecipitated partners under native conditions; the E3 ligase activity may be context-dependent.
• For ELISA, optimize coating and detection conditions using recombinant CBL protein as standard.
• Cross-reactivity with mouse and rat allows use in rodent model studies; but confirm sequence homology in the epitope region for reliable detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our portfolio includes validated monoclonal and polyclonal antibodies, such as this CBL Rabbit mAb, which is ideal for signal transduction and oncology research applications. Beyond individual reagents, we offer bulk ancillary reagents, antibody pairs, and rare target sourcing to streamline your IVD development.