Anti-CBFB Rabbit Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - Q13951

Core Product Specifications and Parameters

Biological Background: CBFB Function and Localization

• Encoded by the CBFB gene, core-binding factor subunit beta is a transcription factor subunit that forms a heterodimeric complex with RUNX family proteins (RUNX1, RUNX2, RUNX3) known as core-binding factor (CBF). CBFB allosterically enhances the DNA-binding affinity of RUNX transcription factors.
• CBF complexes bind to the core consensus sequence 5′-TGTGGT-3′ in enhancers and promoters of various genes, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3, and GM-CSF, thereby regulating transcription.
• During cytotoxic (CD8+) T cell development, CBF complexes act as transcriptional silencers by binding to the RUNX-binding sequence within the ZBTB7B locus, repressing its expression to allow differentiation.
• CBFB is targeted by HIV-1 Vif protein, which recruits it to form a Cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex) that catalyzes the ubiquitination and degradation of APOBEC3F and APOBEC3G (PubMed:22190037, PubMed:31792451, PubMed:36598981). The complex can also ubiquitinate APOBEC3H to some extent (PubMed:37640699). Vif association also inhibits the coactivator activity of CBFB (PubMed:22190037). Related references: PMID:22190037 PMID:31792451 PMID:36598981 PMID:37640699
• Subcellular localization is predominantly nuclear, consistent with its role in transcriptional regulation.
• Keywords from UniProt highlight its involvement in alternative splicing, chromosomal rearrangements (e.g., inv(16) in acute myeloid leukemia), proto-oncogenic potential, and disease associations including dwarfism. It is also a phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering amino acids 1-182 of human CBFB. This N-terminal portion contains the conserved domain necessary for RUNX interaction and is identical or highly similar in mouse and rat, supporting the observed cross-reactivity.
• For western blot (WB), a band near 22 kDa is expected. Consider using nuclear extracts and appropriate loading controls.
• Immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P) should reveal nuclear localization. Validate in known CBFB-expressing cell lines or tissues.
• ELISA can be used for quantitative analysis; optimal coating conditions and antibody concentrations should be determined empirically.
• As with any antibody, validate in the specific experimental context and consider pre-adsorption controls if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For CBFB-related research and assay development, we supply high-quality monoclonal antibodies like this CBFB Rabbit mAb, along with validated antibody pairs, optimized reagents, and bulk ancillary materials to support your workflow.