Anti-CBFA2T3 Rabbit Polyclonal Antibody for WB, ELISA - O75081

Core Product Specifications and Parameters

Biological Background: CBFA2T3 Function and Localization

• CBFA2T3 (also known as ETO2, MTG16, MTGR2, ZMYND4, RUNX1T3) is a transcriptional corepressor that mediates repression via association with DNA-binding transcription factors and recruitment of histone-modifying enzymes. Related references: PMID:12559562, PMID:15203199, PMID:16966434
• It plays a role in regulating glycolysis and mitochondrial respiration by downregulating HIF1A target genes; it promotes HIF1A degradation through prolyl hydroxylation-dependent ubiquitination. Related references: PMID:25974097, PMID:23840896
• CBFA2T3 contributes to erythroid progenitor proliferation and differentiation by repressing TAL1 target genes.
• It is involved in granulocyte differentiation. Related references: PMID:15231665
• Isoform 2 functions as an A-kinase-anchoring protein. Related references: PMID:11823486
• The protein localizes to the nucleus, nucleolus, nucleoplasm, and Golgi apparatus membrane.
• CBFA2T3 is widely expressed, with higher levels in heart, pancreas, skeletal muscle, spleen, thymus, and peripheral blood leukocytes, and is detected in hematopoietic cells at the protein level.
• It contains zinc-finger domains, is a phosphoprotein, and belongs to the MTG8 family of transcriptional regulators.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 593-653), which may help in specific detection of full-length or cleaved forms of CBFA2T3.
• For Western blot, use standard reducing conditions; expect a band near 71 kDa. Include positive control cell lines such as HeLa or Jurkat, which express CBFA2T3.
• This antibody is predicted to cross-react with mouse and rat, so it is suitable for studies in rodent models.
• In ELISA applications, pair with an appropriate detection reagent; optimal dilutions should be determined empirically for your assay system.
• Polyclonal antibodies may exhibit lot-to-lot variation; consider validating each new batch with a consistent set of reference samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in streamlining IVD raw material procurement for diagnostic manufacturers and research laboratories. For transcriptional regulation and cancer biology projects involving CBFA2T3, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our team can also assist with sourcing rare target raw materials to accelerate your development from concept to clinic.