Anti-CBFA2T2 Polyclonal Antibody for WB, IF/ICC, ELISA - O43439

Core Product Specifications and Parameters

Biological Background: CBFA2T2 Function and Localization

• Protein CBFA2T2 (EHT/MTGR1/ZMYND3/p85) acts as a transcriptional corepressor by recruiting histone‑modifying enzymes and other corepressors to DNA‑binding transcription factors. Related references: PMID:12559562 PMID:15203199
• Through interaction with PRDM14, CBFA2T2 stabilizes PRDM14 and OCT4 on chromatin, playing a key role in embryonic stem cell (ESC) pluripotency and primordial germ cell (PGC) formation. Related references: PMID:27281218
• In a ZBTB33‑dependent manner, CBFA2T2 represses the expression of MMP7. Related references: PMID:23251453
• In acute myeloid leukemia, CBFA2T2 may function in a complex with the RUNX1‑CBFA2T1 fusion protein, contributing to the repression of AML1‑dependent transcription and induction of G‑CSF/CSF3‑dependent cell growth.
• Through heteromerization with CBFA2T3/MTG16, it helps regulate erythroid progenitor proliferation and differentiation by repressing TAL1 target genes.
• CBFA2T2 is required for maintaining the secretory cell lineage in the small intestine; it can inhibit Notch signaling via association with RBPJ and may participate in GFI1‑mediated Paneth cell differentiation.
• The protein contains coiled‑coil and zinc‑finger domains, undergoes phosphorylation and ubiquitination, and is localized exclusively in the nucleus.
• Widely expressed in fetal and adult tissues, with notably high levels in brain, heart, lung, kidney, lymphoid organs, and reproductive tissues.

Experimental Guidance and Technical Tips

• Use this antibody for Western blot detection of endogenous CBFA2T2; expect a band near 67 kDa. Validate the specificity in your sample by using appropriate controls.
• For immunofluorescence/immunocytochemistry (IF/ICC), CBFA2T2 localizes to the nucleus; optimize fixation and permeabilization conditions for your cell type.
• This antibody is suitable for ELISA; the recombinant immunogen (amino acids 325–604) can serve as a positive control or coating antigen.
• When blotting or staining tissues, note that CBFA2T2 is ubiquitously expressed but highly abundant in brain, heart, lung, kidney, and lymphoid tissues; mouse and rat models are appropriate cross-reactive species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined sourcing channel for IVD manufacturers and researchers requiring high-quality raw materials. This anti-CBFA2T2 polyclonal antibody is part of a broader portfolio that includes validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents to support your immunodetection assay development. Our team can also assist with rare target raw-material procurement, helping you bridge from concept to clinical application.