Anti-Caveolin-3 Polyclonal Antibody for WB, IHC-P, ELISA - P56539

Core Product Specifications and Parameters

Biological Background: Caveolin-3 Function and Localization

• Caveolin-3, also known as M-caveolin, is a scaffolding protein predominantly expressed in muscle tissues, where it localizes to caveolae — small invaginations of the plasma membrane.
• It functions as a molecular scaffold within caveolar membranes, directly interacting with G-protein alpha subunits and modulating their activity, as well as regulating voltage-gated potassium channels (By similarity).
• Caveolin-3 plays a critical role in sarcolemma repair in both skeletal and cardiac muscle, facilitating rapid membrane resealing after mechanical stress-induced disruption (By similarity).
• It mediates the recruitment of CAVIN2 and CAVIN3 proteins to caveolae, essential for caveola formation and stability. Related references: PMID:19262564
• The protein is localized to the Golgi apparatus membrane and cell membrane, particularly enriched in caveolae and the sarcolemma.
• Mutations in CAV3 are associated with a spectrum of muscle disorders, including limb-girdle muscular dystrophy type 1C (LGMD1C), rippling muscle disease (RMD2), and long QT syndrome type 9 (LQT9), as well as cardiomyopathy.
• Post-translational modifications include isopeptide bond formation and ubiquitin conjugation, which may regulate its stability and function.

Experimental Guidance and Technical Tips

• The immunogen encompasses the full-length human Caveolin-3 (residues 1-151), which should generate antibodies recognizing native and denatured forms; confirm reactivity in the intended sample system.
• For Western blotting, a band at approximately 17 kDa is expected in human and mouse muscle lysates; use appropriate loading controls and consider protein extraction conditions to preserve membrane proteins.
• For immunohistochemistry (paraffin), antigen retrieval and detection conditions may need optimization for different muscle tissue types; include positive and negative controls.
• As a polyclonal antibody, lot-to-lot consistency should be verified for critical quantitative experiments; validated blocking peptides or recombinant protein can be used to confirm specificity.
• The antibody is also suitable for ELISA; assay conditions should be standardized with recombinant Caveolin-3 protein as a reference.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive sourcing service for IVD raw materials, including validated monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing. This anti-Caveolin-3 antibody is well-suited for research in muscle biology, cardiac pathophysiology, and neuromuscular disease diagnostics. Contact our team to integrate this antibody into your diagnostic assay development or to explore custom antibody and reagent supply for scale-up.