Anti-Caveolin-2 Rabbit pAb for WB - P51636

Core Product Specifications and Parameters

Biological Background: Caveolin-2 Function and Localization

• Caveolin-2 (CAV2) is a member of the caveolin family, encoded by the CAV2 gene; it functions as a scaffolding protein within caveolar membranes.
• It directly interacts with G protein alpha subunits, functionally regulating their activity, and works in conjunction with CAV1 to target lipid rafts and drive caveolae formation.
• The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells and acts as a positive regulator of cellular mitogenesis via the MAPK signaling pathway.
• Subcellular localization includes the nucleus, cytoplasm, Golgi apparatus membrane, cell membrane, and caveolae membranes.
• Tissue expression is observed in endothelial cells, smooth muscle cells, skeletal myoblasts, and fibroblasts.
• Relevant UniProt keywords: alternative initiation, alternative splicing, phosphoprotein; the protein is a component of caveolae membrane microdomains.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-65) of human Caveolin-2; consider that the antibody may primarily recognize the N-terminus and potential alternative splicing variants.
• For Western blotting, the predicted molecular weight is ~18 kDa; note that post-translational modifications may affect migration, and include appropriate positive controls such as caveolae-enriched fractions.
• For IHC-P and IF/ICC, optimize permeabilization protocols since Caveolin-2 is associated with membranes and caveolae.
• Validate the antibody in physiologically relevant cell lines expressing Caveolin-2, such as endothelial cells, smooth muscle cells, or fibroblasts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized mono/polyclonal antibodies, and bulk ancillary reagents. This anti-Caveolin-2 rabbit pAb supports research on caveolae biology and MAPK signaling. For niche targets or custom supply needs, our expert team streamlines procurement from concept to clinical application.