Anti-Caveolin-2 Rabbit Monoclonal Antibody for WB, IHC-P, IP, ELISA - P51636

Core Product Specifications and Parameters

Biological Background: Caveolin-2 Function and Localization

• Protein identity and isoforms: Caveolin-2, encoded by the CAV2 gene in human, is a member of the caveolin protein family. Alternative initiation and splicing produce multiple isoforms.
• Core caveolar function: May act as a scaffolding protein within caveolar membranes, directly interacting with G protein alpha subunits and functionally regulating their activity.
• Caveolae formation: Acts as an accessory protein in conjunction with CAV1 to target lipid rafts and drive caveolae formation.
• Mitotic regulation: The Ser-36 phosphorylated form plays a role in modulating mitosis in endothelial cells.
• MAPK signaling: Functions as a positive regulator of cellular mitogenesis through the MAPK signaling pathway, required for insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, thereby regulating cell cycle progression.
• Subcellular localization: Located in the nucleus, cytoplasm, Golgi apparatus membrane, cell membrane, and caveolar membrane.
• Tissue expression: Expressed in endothelial cells, smooth muscle cells, skeletal myoblasts, and fibroblasts.
• Key post-translational modification: Phosphoprotein, with phosphorylation at Ser-36 critical for specific functions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (amino acids 1-100) of human Caveolin-2; ensure the epitope is accessible in your specific assay format and sample type.
• For Western blot (WB), the predicted molecular weight is 18kDa; validate in relevant human or mouse cell lysates (e.g., endothelial or muscle cells) following standard SDS-PAGE and transfer protocols.
• In immunohistochemistry-paraffin (IHC-P), antigen retrieval may be required; consider testing citrate or EDTA-based heat-induced epitope retrieval and confirm specificity with a blocking peptide if available.
• For immunoprecipitation (IP), optimize the antibody-to-lysate ratio and use appropriate lysis buffers that preserve caveolar membrane complexes; a negative control IgG should be included.
• In ELISA applications, a peptide coating antigen can serve as a positive control; titer the antibody concentration to achieve optimal signal-to-noise ratio.
• The antibody cross-reacts with human and mouse; when working with other species, verify sequence homology of the immunogen region and test empirically.

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CamelBio supports diagnostic manufacturers, labs, and research institutes with comprehensive access to IVD raw materials, including validated monoclonal and polyclonal antibodies for targets like Caveolin-2 involved in caveolae biology and signal transduction. Our global supply network delivers optimized antibody pairs, bulk ancillary reagents, and rare target raw materials, providing a seamless bridge from concept to clinic for your assay development and research needs.