Anti-Caveolin-1 Rabbit mAb (KO Validated) for WB, IHC-P, ELISA - Q03135

Core Product Specifications and Parameters

Biological Background: Caveolin-1 Function and Localization

• Caveolin-1 acts as a scaffolding protein within caveolar membranes, organizing signaling molecules. Related references: PMID:11751885
• It forms a heterooligomeric complex with CAV2 that targets lipid rafts and drives caveolae formation; it also directly interacts with G protein alpha subunits to regulate their activity.
• Mediates the recruitment of CAVIN proteins (CAVIN1/2/3/4) to caveolae, essential for caveolae biogenesis. Related references: PMID:19262564
• Involved in T-cell receptor-mediated costimulation: binding to DPP4 induces T-cell proliferation and NF-kappa-B activation. Related references: PMID:17287217
• Recruits CTNNB1 to caveolar membranes, potentially regulating Wnt pathway signaling (By similarity); binds 20(S)-hydroxycholesterol (By similarity).
• Negatively regulates TGFB1 signaling by internalizing TGFBR1 from membrane rafts, leading to SMAD2/3 pathway inhibition. Related references: PMID:25893292
• Localizes to the Golgi apparatus, cell membrane, caveolae, membrane rafts, and cytoplasm.
• Expressed in skeletal muscle, liver, stomach, lung, kidney, heart, and brain.

Experimental Guidance and Technical Tips

• Based on the immunogen design (a.a. 1-80 of human Caveolin-1, NP_001744.2), the antibody likely targets the N-terminus, which is conserved across isoforms. For WB, validate using lysates from human, mouse, or rat tissues; expect bands around 17–20 kDa.
• In IHC-P, consider standard antigen retrieval methods and use positive controls such as human liver or skeletal muscle sections.
• For ELISA, evaluate binding with recombinant Caveolin-1 or caveolae-rich membrane preparations.
• Given the reported cross-reactivity with mouse and rat, the antibody may be suitable for multi-species studies; verify in the relevant model system.
• The antibody's application in studying caveolae biology, T-cell signaling, and TGF-β pathway regulation may be explored.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop access to IVD raw materials, offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents for diagnostic development. This anti-Caveolin-1 antibody supports research in membrane biology and cell signaling, and CamelBio can assist with sourcing rare-target raw materials and custom antibody production for your diagnostic pipeline.