Anti-Caveolin-1 Polyclonal Antibody for IF/ICC and ELISA - Q03135

Core Product Specifications and Parameters

Biological Background: Caveolin-1 Function and Localization

• Caveolin-1 (CAV1) is a scaffolding protein within caveolar membranes, playing a key role in caveolae formation by forming heterooligomeric complexes with CAV2 and recruiting CAVIN proteins (CAVIN1/2/3/4). Related references: PMID:11751885, PMID:19262564
• It interacts directly with G protein alpha subunits, functionally regulating their activity, and is involved in T-cell receptor-mediated T-cell activation; binding to DPP4 induces T-cell proliferation and NF-kappa-B activation. Related references: PMID:17287217
• CAV1 negatively regulates TGFB1-mediated signaling by mediating internalization of TGFBR1 from membrane rafts, leading to receptor degradation. Related references: PMID:25893292
• The protein localizes to the Golgi apparatus membrane, cell membrane, caveolae, membrane rafts, and cytoplasm, highlighting its dynamic subcellular distribution.
• It is expressed in skeletal muscle, liver, stomach, lung, kidney, heart, and brain at the protein level.
• Keywords include acetylation, palmitoylation, phosphorylation, and ubiquitination, indicating extensive post-translational modifications, with a molecular mass of ~20 kDa.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1–100) of human Caveolin-1, which may confer specificity for detecting the target in IF/ICC and ELISA applications.
• For IF/ICC, consider optimizing fixation and permeabilization protocols to preserve caveolar structures, as Caveolin-1 is membrane-associated.
• In ELISA, validate the antibody with appropriate recombinant protein or cell lysates, and include negative controls to confirm specificity.
• Polyclonal antibodies may exhibit lot-to-lot variation; consistent performance should be verified in the intended assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated sourcing partner for IVD raw materials, from concept to clinic. This Caveolin-1 polyclonal antibody supports research in membrane biology, caveolae function, and related signaling pathways. Beyond antibodies, we supply validated antibody pairs, bulk reagents, and custom sourcing for rare targets to streamline your diagnostic development.