Anti-Caveolin-1 Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - Q03135

Core Product Specifications and Parameters

Biological Background: Caveolin-1 Function and Localization

• Caveolin-1 is the principal scaffolding protein of caveolae, forming a heterooligomeric complex with CAV2 that localizes to lipid rafts and is essential for caveolae biogenesis. Related references: PMID:11751885
• It recruits the CAVIN complex (CAVIN1/2/3/4) to caveolae, further stabilizing the invaginated structure. Related references: PMID:19262564
• Caveolin-1 directly interacts with G-protein alpha subunits and modulates their activity, linking caveolae to G-protein-coupled receptor signaling.
• It acts as a co-stimulatory molecule in T-cell receptor-mediated activation by binding DPP4, triggering T-cell proliferation and NF-kappa-B activation. Related references: PMID:17287217
• Caveolin-1 negatively regulates TGF-beta signaling by promoting the internalization and degradation of TGFBR1 from membrane rafts. Related references: PMID:25893292
• The protein is localized to the Golgi apparatus, cell membrane, caveolae, and membrane rafts, with cytoplasmic distribution also reported.
• Caveolin-1 is expressed in a variety of tissues including skeletal muscle, liver, stomach, lung, kidney, heart, and brain.
• Post-translational modifications include palmitoylation, acetylation, phosphorylation, and ubiquitination; it is associated with diseases such as congenital generalized lipodystrophy and diabetes mellitus.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-80), encompassing functionally important domains. For Western blot, anticipate a band at approximately 17–20 kDa due to alternative initiation isoforms; validate in the relevant sample system.
• This monoclonal antibody is suitable for WB, IF/ICC, IHC-P, and ELISA. For immunofluorescence, expect distinct membrane and vesicular staining patterns. For IHC on paraffin sections, antigen retrieval may be necessary.
• As a mouse-derived antibody, use appropriate anti-mouse secondary reagents and include isotype controls when required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop supplier of IVD raw materials, supporting diagnostic manufacturers from R&D to production. We provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for high-demand targets like Caveolin-1. Our expertise in molecular biology and immunodiagnostics enables rare-target sourcing and custom development capabilities for your clinical assay needs.