Anti-Cation-independent M6PR (IGF2R) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P11717

Core Product Specifications and Parameters

Biological Background: Cation-independent M6PR (IGF2R) Function and Localization

• Cation-independent M6PR (IGF2R), also designated 300 kDa mannose 6-phosphate receptor (MPR300) or insulin-like growth factor 2 receptor, is a large transmembrane glycoprotein encoded by the IGF2R gene.
• It mediates the transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to the receptor, and the receptor-ligand complex is internalized, dissociating in the acidic pre-lysosomal compartment. The receptor is then recycled back to the Golgi via the retromer complex. Related references: PMID:18817523, PMID:2963003
• In addition to its M6P-dependent trafficking role, IGF2R binds insulin-like growth factor 2 (IGF2) with high affinity, functioning as a clearance receptor that regulates extracellular IGF2 levels. Related references: PMID:18046459
• IGF2R acts as a positive regulator of T-cell coactivation by binding dipeptidyl peptidase 4 (DPP4). Related references: PMID:10900005
• The receptor cycles dynamically between the Golgi apparatus membrane and endosomal compartments, a localization essential for its sorting and transport functions.
• IGF2R is extensively post-translationally modified, featuring glycosylation, disulfide bonds, acetylation, methylation, palmitoylation, and phosphorylation. Its structure includes a signal peptide, 15 extracytoplasmic repeats, a single transmembrane helix, and a short cytoplasmic tail.
• The mature protein has a predicted molecular weight of approximately 274 kDa, consistent with its designation as MPR300.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 2140-2300), which may favor detection of full-length IGF2R. Consider using lysosome-rich tissue or cell line lysates (e.g., liver, placenta, or fibroblasts) as positive controls for Western blotting.
• Due to the large protein size (274 kDa), use a low-percentage (6–8%) polyacrylamide gel and extended wet transfer conditions (e.g., overnight at 4°C) onto a PVDF membrane. Include pre-stained high molecular weight markers to monitor transfer efficiency.
• For IHC-P, perform appropriate antigen retrieval and optimize primary antibody dilution. Include positive and negative tissue controls to validate staining specificity in both human and rat samples.
• ELISA applications may benefit from recombinant protein standards to establish quantitative assays. If experimental designs involve rodent models, validate cross-reactivity in rat systems.
• Always validate the antibody in your specific experimental context, as performance may vary with sample preparation and assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, research laboratories, and institutions with one-stop access to high-quality IVD raw materials, technical services, and consulting from concept to clinic. This anti-Cation-independent M6PR (IGF2R) antibody exemplifies our commitment to supplying validated monoclonal antibodies for challenging targets in lysosomal biology and growth factor signaling. Beyond single reagents, we offer bulk ancillary reagents, rare-target sourcing, and customized solutions to streamline your assay development pipeline.