Anti-Cation-independent M6PR (IGF2R) Monoclonal Antibody for WB, IF/ICC, ELISA - P11717

Core Product Specifications and Parameters

Biological Background: Cation-independent M6PR (IGF2R) Function and Localization

• Encoded by the IGF2R gene, the cation-independent mannose-6-phosphate receptor (CI-M6PR) is also known as MPR300, CD222, and insulin-like growth factor 2 receptor (IGF2R), with a molecular weight of approximately 274 kDa.
• Mediates the transport of newly synthesized lysosomal enzymes bearing phosphomannosyl residues from the Golgi complex to lysosomes, as well as the endocytosis of extracellular enzymes from the cell surface (PubMed:18817523, PubMed:2963003).
  Related references: PMID:18817523 PMID:2963003
• The receptor-ligand complex dissociates in the acidic environment of a prelysosomal compartment, allowing the receptor to recycle back to the Golgi via retromer binding (PubMed:18817523).
  Related references: PMID:18817523
• Functions as a receptor for insulin-like growth factor 2 (IGF2), with binding contributing to the regulation of IGF2 levels (PubMed:18046459).
  Related references: PMID:18046459
• Positively regulates T-cell coactivation through interaction with dipeptidyl peptidase 4 (DPP4) (PubMed:10900005).
  Related references: PMID:10900005
• Localizes primarily to the Golgi apparatus membrane and the endosome membrane, consistent with its role in intracellular trafficking.
• Post-translational modifications include glycosylation, disulfide bonds, palmitoylation, acetylation, and phosphorylation, which may influence receptor stability and function.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the C-terminal region (aa 2392-2491), which may provide specificity for the full-length receptor.
• For Western blotting, the high molecular weight (274 kDa) necessitates the use of low-percentage (e.g., 6-8%) SDS-PAGE gels, extended transfer times, and appropriate molecular weight markers.
• In IF/ICC, permeabilization steps should be optimized to detect the receptor at Golgi and endosome membranes, as its localization is intracellular.
• Cross-reactivity with mouse and rat orthologs has been observed; validation in the relevant species and tissue type is recommended.
• ELISA applications may require titration of the antibody to suit the assay format and target coating conditions.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material supplier, CamelBio offers validated monoclonal antibodies like this anti-CI-M6PR (IGF2R) clone, along with antibody pairs, recombinant proteins, and ancillary reagents to support your M6PR/IGF2R-related diagnostic and research needs. With a portfolio spanning rare targets and bulk quantities, we bridge the gap from concept to clinic with reliable, scalable sourcing solutions.