Anti-Cathepsin S Rabbit Polyclonal Antibody for WB, IF/ICC, IF-P, IHC-P, ELISA - O70370

Core Product Specifications and Parameters

Biological Background: Cathepsin S Function and Localization

• Cathepsin S is a thiol protease. Related references: PMID:26216096
• It is the key protease responsible for the removal of the invariant chain from MHC class II molecules, enabling MHC class II antigen presentation.
• Its bond specificity partially resembles that of cathepsin L.
• Cathepsin S elicits itch by mediating the cleavage and activation of the receptor MRGPRX1. Related references: PMID:26216096
• The enzyme is localized in lysosomes, and can be secreted or found in cytoplasmic vesicles and phagosomes.
• It is widely expressed, with highest levels in non-skeletal tissues, and is present in spleen, B cells, dendritic cells, and macrophages. Related references: PMID:9545226
• Cathepsin S is synthesized as a zymogen and contains a signal peptide, disulfide bonds, and glycosylation sites.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the region spanning the propeptide and mature enzyme; consider validating the antibody's reactivity against both pro and mature forms of Cathepsin S in your sample system.
• For Western blot, a band at approximately 38 kDa may be observed; post-translational modifications or activation cleavage could result in additional bands.
• This antibody has been used in immunofluorescence and immunohistochemistry; optimize fixation and permeabilization conditions for your specific tissue or cell type.
• Suitable for ELISA; consider using a blocking buffer that minimizes non-specific binding of rabbit antibodies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides IVD manufacturers and research laboratories with comprehensive access to raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target sourcing services. This anti-Cathepsin S antibody supports research in antigen presentation and protease biology, and CamelBio facilitates the efficient procurement of all related reagents to accelerate your diagnostic development.