Anti-Cathepsin L Monoclonal Antibody for WB, IHC-P, ELISA - P07711

Core Product Specifications and Parameters

Biological Background: Cathepsin L Function and Localization

• Procathepsin L (Cathepsin L1/MEP) is a lysosomal thiol protease that plays a central role in protein degradation, antigen processing, and bone remodeling (Probable).
• Alternative translation initiation produces nuclear isoforms that cleave the CUX1 transcription factor, regulating cell cycle progression. Related references: PMID:15099520
• In the thyroid, it solubilizes thyroglobulin for T4 hormone release; in neuroendocrine cells, it processes proenkephalin to enkephalin.
• Secreted Cathepsin L generates endostatin from collagen XVIII, and as an elastase, it degrades extracellular matrix in inflammation. Related references: PMID:10716919
• Facilitates entry of SARS-CoV, SARS-CoV-2, and other viruses by proteolytically activating spike glycoproteins in lysosomes. Related references: PMID:32142651, PMID:16339146, PMID:18562523
• Subcellularly, it is found in lysosomes, secretory vesicles, apical cell membrane, extracellular space, and nucleus; active as a mature enzyme after proteolytic removal of the propeptide.
• Belongs to the papain family (C1) of cysteine proteases; it is a glycoprotein with disulfide bonds, synthesized as a zymogen and activated by low pH or other proteases.

Experimental Guidance and Technical Tips

• The immunogen (aa 100–200) is located in the N-terminal region of human procathepsin L; the epitope may be exposed in both the proenzyme and mature enzyme, but users should confirm specificity for the desired form.
• For Western blot, the primary band is expected at ~38 kDa (procathepsin L); under reducing conditions, the mature heavy chain (~25 kDa) may also be detected. Optimize loading and lysis conditions to minimize autolysis.
• In IHC-P, use heat-induced antigen retrieval (e.g., citrate buffer pH 6.0) and validate with positive tissues known to express Cathepsin L (e.g., liver, placenta). Include isotype control.
• For ELISA, consider pairing with a different monoclonal or polyclonal antibody to the target to build a sandwich assay, and validate linearity and recovery in your matrix.

CamelBio: Your One-Stop Sourcing Bridge

For researchers developing assays for Cathepsin L—a key player in cancer invasion, immune function, and SARS-CoV-2 infection—CamelBio offers reliable monoclonal antibodies, matched antibody pairs, and bulk production support. Our one-stop sourcing platform extends to specialized ancillary reagents and hard-to-find targets, enabling seamless transition from development to commercial IVD kits.