Anti-Cathepsin H Rabbit Monoclonal Antibody for WB, ELISA - P09668

Core Product Specifications and Parameters

Biological Background: Cathepsin H Function and Localization

• Cathepsin H is encoded by the CTSH gene (also known as CPSB) and exists as a pro-enzyme (pro-cathepsin H) that is proteolytically processed into its mature, active form.
• It functions as a lysosomal cysteine protease, playing a key role in the overall degradation of proteins within the lysosome.
• Cathepsin H belongs to the papain family of cysteine proteases and exhibits both endopeptidase and aminopeptidase activities.
• The protein is characterized as a thiol protease, relying on a cysteine residue in its active site for catalytic activity.
• It is synthesized as a zymogen (pro-cathepsin H) and undergoes post-translational modifications including glycosylation and disulfide bond formation, which are critical for proper folding and stability.
• Cathepsin H is predominantly localized to the lysosome, consistent with its role in intracellular protein turnover.
• As evidenced by keywords such as "Glycoprotein" and "Disulfide bond," its structure includes carbohydrate modifications and stabilizing disulfide linkages.

Experimental Guidance and Technical Tips

• For Western blot, target band detection around 37 kDa corresponding to pro-cathepsin H; note that processed forms may appear at lower molecular weights.
• Given the recombinant immunogen spanning a conserved region, validate antibody specificity in relevant human and mouse tissue or cell lysates to confirm expected reactivity.
• For ELISA applications, consider pairing this monoclonal antibody with a suitable detection reagent; assess optimal capture and detection conditions empirically.
• Since cathepsin H contains disulfide bonds, use reducing sample buffer for SDS-PAGE to ensure complete denaturation.
• The antibody's reactivity with mouse samples allows cross-species validation in preclinical models; nonetheless, confirm consistent performance prior to study initiation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw material procurement, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-Cathepsin H antibody supports research in lysosomal degradation and protease-related disorders, and we can assist with sourcing additional rare-target raw materials to streamline your assay development. From concept to clinic, CamelBio bridges the gap with comprehensive supply solutions tailored to diagnostic innovators.