Anti-Cathepsin G Rabbit Monoclonal Antibody for WB, ELISA - P08311

Core Product Specifications and Parameters

Biological Background: Cathepsin G Function and Localization

• Cathepsin G is a serine protease with chymotrypsin-like specificity, preferring Phe and Tyr at the P1 position. Related references: PMID:29652924, PMID:8194606
• It exhibits direct antimicrobial activity against Gram-positive and Gram-negative bacteria, degrading biofilms and facilitating phagocytosis. Related references: PMID:2116408, PMID:2117044
• Cathepsin G mediates platelet activation via PAR4 and modulates thrombin receptor PAR1 signaling. Related references: PMID:10702240, PMID:3390156, PMID:7744748
• The protease binds to and activates B cells, T cells, and NK cells, enhancing cytotoxicity. Related references: PMID:7842483, PMID:9000539, PMID:9536127
• It localizes to neutrophil granules, is secreted, and also detected in the nucleus, lysosome, and plasma membrane.
• Expressed primarily in neutrophils and B cells. Related references: PMID:3799965, PMID:15100291
• Cathepsin G processes multiple immune substrates, including complement C3, IL-33, and IL-36γ, to regulate inflammation. Related references: PMID:1861080, PMID:22307629, PMID:30804664
• During S. aureus infection, bacterial Eap proteins inhibit Cathepsin G, reducing neutrophil microbicidal activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 156–255 of human Cathepsin G, suggesting the antibody recognizes an internal epitope conserved in the mature protease. Consider antigen retrieval or sample preparation methods appropriate for your applications.
• For Western Blot, human neutrophil or T-cell lysates are suitable positive controls; expect a band at approximately 29 kDa (mature form). Optimize antibody dilution (e.g., 1:500–1:2000) and validate with a known Cathepsin G-positive sample.
• In ELISA, the antibody can serve as a detection reagent; pair with a capture antibody recognizing a distinct epitope. Titrate the antibody to achieve optimal signal-to-noise ratio.
• The recombinant rabbit monoclonal format offers high specificity and lot-to-lot reproducibility, reducing variability in long-term studies.
• Cross-reactivity with species other than human has not been evaluated; perform independent validation if working with non-human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted bridge for IVD raw-material sourcing, providing a comprehensive portfolio including validated antibody pairs, bulk monoclonal antibody reagents, and rare target sourcing. Whether you require Cathepsin G antibodies for immunoassay development or ancillary reagents for neutrophil biology research, our one-stop supply chain supports every stage from concept to clinic. Partner with CamelBio to streamline procurement and accelerate your diagnostic assay development.