Anti-Cathepsin E (CTSE) Monoclonal Antibody for WB, ELISA - P14091

Core Product Specifications and Parameters

Biological Background: Cathepsin E Function and Localization

• Cathepsin E is an aspartyl protease belonging to the pepsin family, encoded by the CTSE gene; alternative splicing yields multiple isoforms.
• It plays a role in immune function, likely participating in antigenic peptide processing during MHC class II-mediated antigen presentation.
• In the thymus, it may contribute to activation-induced lymphocyte depletion; in the brain, it has been implicated in neuronal degeneration and glial cell activation.
• The protein localizes primarily to endosomes.
• Expression is abundant in the stomach, Clara cells of the lung, and activated B-lymphocytes, with lower levels in lymph nodes, skin, and spleen; it is absent from resting B-lymphocytes.
• Synthesized as an inactive zymogen, it undergoes autocatalytic cleavage to become active; the mature enzyme is a glycoprotein with disulfide bonds.
• The molecular weight of the mature enzyme is approximately 43 kDa (precursor ~42.8 kDa).

Experimental Guidance and Technical Tips

• The immunogen (aa 132-244 of human CTSE) likely targets the mature enzyme; consider using the antibody to detect both precursor and active forms.
• For Western blot, expect a band near 43 kDa under reducing conditions; use positive controls such as stomach or lung tissue lysates.
• When performing ELISA, validate conditions with recombinant protein or lysates from tissues expressing CTSE.
• Cross-reactivity with mouse and rat enables use in rodent models for studying immune response or neurodegeneration.
• As cathepsin E activity is optimal at acidic pH, sample preparation and buffer choice may influence detection; consider optimizing lysis buffers.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive one-stop sourcing partner for IVD raw materials, connecting diagnostic manufacturers and research institutes with high-quality reagents. For studies on cathepsin E in immune function and antigen presentation, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support your entire workflow. From routine catalog products to rare target raw material sourcing, CamelBio’s extensive supply network ensures a reliable path from concept to clinic.