Anti-Cathepsin D Rabbit Polyclonal Antibody for WB, ELISA - P07339

Core Product Specifications and Parameters

Biological Background: Cathepsin D Function and Localization

• Cathepsin D is an aspartyl protease encoded by the CTSD gene.
• It functions as an acid protease active in intracellular protein breakdown within lysosomes.
• It plays a role in amyloid precursor protein (APP) processing following cleavage and activation by ADAM30, leading to APP degradation. Related references: PMID:27333034
• Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
• Subcellular localization includes lysosomes, melanosomes, and it can be secreted into the extracellular space.
• Expressed in the aorta extracellular space and liver at the protein level.
• Translation yields a preproenzyme; mature Cathepsin D is a glycoprotein with a disulfide bond.
• Keywords: Aspartyl protease, Hydrolase, Neuronal ceroid lipofuscinosis, Neurodegeneration.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 65-412, which covers the mature form of Cathepsin D after proteolytic processing. Consider this when designing detection strategies.
• For Western blotting, the predicted molecular weight of the precursor is approximately 45 kDa, but mature and intermediate forms may appear at lower molecular weights due to processing. Validate in your relevant sample system.
• When using this polyclonal antibody in ELISA, consider appropriate positive and negative controls and confirm performance with your specific assay conditions.

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CamelBio provides comprehensive access to IVD raw materials, supporting researchers and diagnostic manufacturers from early-stage development to clinical application. Our portfolio includes validated antibody pairs, optimized polyclonal antibodies like this anti-Cathepsin D, and bulk ancillary reagents for protein degradation, Alzheimer disease, and cancer research. We also assist with rare-target sourcing—contact us to discuss your specific project needs.