Anti-Cathepsin D Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P07339

Core Product Specifications and Parameters

Biological Background: Cathepsin D Function and Localization

• Cathepsin D is an aspartyl protease encoded by the CTSD gene, initially produced as an inactive zymogen that undergoes proteolytic processing to its mature form.
• It functions as a major acid protease responsible for intracellular protein turnover and plays a role in APP processing after activation by ADAM30, promoting APP degradation. Related references: PMID:27333034
• The protease is implicated in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer’s disease.
• At the subcellular level, Cathepsin D localizes predominantly to lysosomes, but is also found in melanosomes and can be secreted into the extracellular space.
• Tissue expression is notable in the aorta extracellular space and liver, as demonstrated at the protein level. Related references: PMID:20551380 PMID:1426530
• Post-translational modifications include N-linked glycosylation and formation of disulfide bonds essential for its structure; the protein is a member of the peptidase A1 family.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C‑terminal region (amino acids 313‑412) of human Cathepsin D, which may recognize both pro‑ and mature forms; consider this when analyzing processed variants.
• For Western blotting, a band around 45 kDa is predicted, but Cathepsin D undergoes processing that can yield multiple species; validate the observed pattern in your specific sample system.
• In IHC‑P, staining intensity and localization can vary with fixation and antigen retrieval conditions; optimization is recommended for each tissue type.
• For ELISA applications, ensure that the coating antigen contains the peptide sequence used for immunization to favor optimal antibody capture.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined pathway to high‑quality IVD raw materials, from validated antibody pairs and optimized monoclonal antibodies to bulk ancillary reagents. For researchers targeting Cathepsin D in cancer or neurodegenerative disease diagnostics, our team can assist with sourcing rare‑target specificities and scalable raw materials, supporting your project from early development to clinical application.