Anti-Cathepsin D Polyclonal Antibody for WB, IHC-P, ELISA - P07339

Core Product Specifications and Parameters

Biological Background: Cathepsin D Function and Localization

• Cathepsin D functions as an acid protease essential for intracellular protein catabolism within lysosomes.
• It participates in the processing of amyloid precursor protein (APP), where cleavage by ADAM30 activates cathepsin D to degrade APP, potentially influencing Alzheimer’s disease pathology. Related references: PMID:27333034
• The enzyme is implicated in the pathogenesis of breast cancer and neurodegenerative disorders such as Alzheimer’s disease.
• Cathepsin D is predominantly localized to lysosomes, but it is also found in melanosomes and can be secreted into the extracellular space.
• At the protein level, cathepsin D is expressed in the aorta extracellular space and in the liver. Related references: PMID:20551380 PMID:1426530
• It is synthesized as an inactive zymogen (procathepsin D) and undergoes proteolytic processing to yield the mature active form, a glycoprotein with a molecular weight of approximately 45 kDa.
• Keywords associated with cathepsin D include aspartyl protease, lysosome, neurodegeneration, and neuronal ceroid lipofuscinosis, reflecting its critical roles in health and disease.

Experimental Guidance and Technical Tips

• The immunogen spans the propeptide and part of the mature enzyme (aa 21-249), which could allow detection of both procathepsin D and its processed forms.
• For Western blotting, liver or aorta lysates may serve as suitable positive controls given the reported tissue expression.
• When performing immunohistochemistry (IHC-P), consider optimizing antigen retrieval and antibody dilution using known positive tissue sections.
• For ELISA applications, pairing this antibody with a capture antibody targeting a distinct epitope is recommended to set up a sandwich assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers with a comprehensive portfolio of IVD raw materials. From validated antibody pairs to optimized polyclonal antibodies like this Cathepsin D pAb, we streamline procurement for projects in cancer, neurodegeneration, and lysosomal storage diseases. Our one-stop platform also provides bulk ancillary reagents and custom sourcing for rare targets.