Anti-Cathepsin B Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P07858

Core Product Specifications and Parameters

Biological Background: Cathepsin B Function and Localization

• Cathepsin B (CTSB) is a lysosomal thiol protease that participates in intracellular protein degradation and turnover. Related references: PMID:12220505
• It functions as an APP secretase (APPS) and is also known as Cathepsin B1.
• Cleaves matrix extracellular phosphoglycoprotein MEPE, contributing to bone matrix regulation. Related references: PMID:12220505
• Implicated in tumor invasion and metastasis, highlighting its role in cancer progression. Related references: PMID:3972105
• Involved in solubilization of cross-linked thyroglobulin in the thyroid follicle lumen (By similarity).
• Localizes primarily to lysosomes, but also found in melanosomes, secreted into the extracellular space, and at the apical cell membrane.
• Expressed in the stratum spinosum of the epidermis, with weak detection in the stratum granulosum.
• Key post-translational modifications include disulfide bonds, glycosylation, and acetylation; synthesized as a zymogen.

Experimental Guidance and Technical Tips

• The immunogen (aa 27-156) covers the mature protease domain, likely recognizing both pro- and active Cathepsin B forms; consider this when designing assays for zymogen activation studies.
• For Western blotting, the predicted molecular weight is approximately 38 kDa, but note that disulfide bond patterns and glycosylation may affect migration; reducing and non-reducing conditions can help characterize forms.
• In IHC-P, lysosomal localization often requires optimized antigen retrieval; validate using known positive tissues such as liver or tumor samples.
• For ELISA applications, ensure antibody compatibility with the recombinant or native target, and consider potential interference from serum or cellular proteases.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers one-stop sourcing for IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom rare-target development. For Cathepsin B—a critical enzyme in cancer and lysosomal disease research—we supply high-quality rabbit monoclonal antibodies and can support your assay development with tailored reagents and technical consultation. Partner with CamelBio to accelerate your diagnostic projects from concept to clinical application.