Anti-Cathepsin B Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P07858

Core Product Specifications and Parameters

Biological Background: Cathepsin B Function and Localization

• Gene and Protein Identity: Cathepsin B (CTSB, also known as APP secretase, Cathepsin B1, CPSB) is a lysosomal cysteine protease encoded by the CTSB gene on human chromosome 8p23.1. It belongs to the peptidase C1 family.
• Protease Activity: Acts as a thiol protease involved in intracellular protein degradation and turnover. It cleaves matrix extracellular phosphoglycoprotein (MEPE) and participates in thyroglobulin solubilization in the thyroid.
  Related references: PMID:12220505
• Role in Disease: Implicated in tumor invasion and metastasis, contributing to extracellular matrix remodeling.
  Related references: PMID:3972105
• Subcellular Localization: Primarily localized to lysosomes, but also found in melanosomes, secreted into the extracellular space, and associated with the apical cell membrane in some cell types.
• Tissue Expression: Expressed in the stratum spinosum of the epidermis, with weak expression in the stratum granulosum.
• Post-translational Modifications and Keywords: The protein undergoes acetylation, glycosylation, and disulfide bond formation. It is synthesized as an inactive zymogen and activated by proteolytic cleavage. Keywords include hydrolase, protease, thiol protease, and secreted.

Experimental Guidance and Technical Tips

• Immunogen Design: The immunogen is a recombinant fusion protein spanning amino acids 18–339 of human Cathepsin B (NP_001899.1), covering the mature enzyme sequence. This may enable recognition of both pro- and active forms. Users should consider that the antibody might detect multiple bands corresponding to the proenzyme (~38 kDa) and the mature heavy chain (~25–30 kDa) under reducing conditions.
• Application Notes: This antibody is listed for WB, IHC-P, IF/ICC, and ELISA. For each application, appropriate sample preparation and optimization are recommended. For WB, expect bands near 38 kDa (pro-cathepsin B) or processed forms; for IHC-P and IF/ICC, antigen retrieval and permeabilization methods should be validated in the relevant sample system. ELISA performance depends on coating antigen and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports your IVD development by offering this anti-Cathepsin B polyclonal antibody alongside a full portfolio of companion raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. Leveraging our one-stop sourcing platform, we help streamline procurement for cancer research, protease studies, and diagnostic assay development from concept to clinic.