Anti-Cathepsin B Monoclonal Antibody for WB, IF/ICC, ELISA - P07858

Core Product Specifications and Parameters

Biological Background: Cathepsin B Function and Localization

• Cathepsin B, encoded by the CTSB gene, is a lysosomal cysteine protease belonging to the papain family. Alternative names include APP secretase (APPS) and Cathepsin B1.
• It functions as a thiol protease that cleaves matrix extracellular phosphoglycoprotein MEPE, contributing to intracellular protein degradation and turnover. Related references: PMID:12220505
• Cathepsin B has been implicated in tumor invasion and metastasis, highlighting its role in pathological extracellular matrix remodeling. Related references: PMID:3972105
• By similarity, it participates in solubilizing cross-linked thyroglobulin within the thyroid follicle lumen.
• Subcellularly, Cathepsin B localizes to lysosomes and melanosomes. It can also be secreted into the extracellular space and associates with the apical cell membrane.
• Tissue expression is prominent in the stratum spinosum of the epidermis, with weaker detection in the stratum granulosum.
• The protein undergoes proteolytic processing from a zymogen to an active enzyme, features disulfide bonds, glycosylation, and acetylation, and its three-dimensional structure has been determined.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 8-339) corresponds to the mature enzyme lacking the signal peptide, suggesting the antibody may recognize both pro- and active Cathepsin B forms. Validation in the relevant sample system is recommended.
• For Western blotting, a band near 38 kDa is expected under reducing conditions; additional bands due to processing (e.g., heavy and light chains of the mature enzyme) may be observed.
• The rabbit monoclonal format typically ensures high specificity and lot-to-lot consistency. Confirm optimal working dilutions for your specific experimental setup.
• For immunofluorescence, consider co-localization studies with lysosomal markers (e.g., LAMP1) and use appropriate fixation/permeabilization protocols.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to premium IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing. For Cathepsin B, a key enzyme in protease-related pathologies and cancer biomarker research, CamelBio offers flexible supply options to accelerate your assay development—from concept to clinic.