Anti-Catalase Rabbit Polyclonal Antibody for WB and ELISA - P04040

Core Product Specifications and Parameters

Biological Background: Catalase Function and Localization

• Catalase catalyzes the degradation of hydrogen peroxide into water and oxygen, protecting cells from oxidative damage. Related references: PMID:7882369
• It also acts as a mitogen, promoting the growth of various cell types including T-cells, B-cells, and myeloid leukemia cells. Related references: PMID:7882369
• The enzyme is localized in the peroxisome matrix, consistent with its role in detoxifying peroxisomal oxidases.
• Catalase is a heme- and iron-binding protein that uses NADP as a cofactor, classifying it as an oxidoreductase and peroxidase.
• It undergoes post-translational modifications including acetylation and phosphorylation, as identified by proteomics studies.
• Its molecular weight is approximately 60 kDa (calculated 59.8 kDa), consistent with the immunogen.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 1–225 of human Catalase, suggesting recognition of the N-terminal region. This epitope may be conserved across human, mouse, and rat as indicated by cross-reactivity data.
• For Western blotting (WB), expect a band at approximately 60 kDa. Consider optimizing blocking and detection conditions to minimize non-specific binding in cell or tissue lysates from human, mouse, or rat samples.
• In ELISA, titrate the antibody against recombinant Catalase or native targets to determine optimal coating and detection concentrations, and validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, technical services, and consulting. Our catalase antibody is part of a broader portfolio for oxidative stress and cell proliferation research, supported by validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing solutions.