Anti-Catalase Monoclonal Antibody for WB, IHC-P, ELISA - P04040

Core Product Specifications and Parameters

Biological Background: Catalase Function and Localization

• Catalase is a peroxisomal enzyme that catalyzes the decomposition of hydrogen peroxide (H2O2) into water and molecular oxygen, safeguarding cells from oxidative damage. Related references: PMID:7882369
• It functions as a homotetramer with each subunit containing a heme group and an iron ion, classifying it as a heme-containing oxidoreductase and peroxidase.
• The enzyme exhibits mitogenic activity, promoting the proliferation of diverse cell types including T-cells, B-cells, melanoma cells, and fibroblasts. Related references: PMID:7882369
• Subcellularly, catalase is localized predominantly to the peroxisome matrix, where it processes H2O2 generated by peroxisomal oxidases.
• Post-translational modifications include acetylation and phosphorylation, and the protein can bind NADP as a cofactor (UniProt Keywords).
• The human catalase protein has a molecular weight of approximately 60 kDa (UniProt: 59756 Da; observed at ~60 kDa in SDS-PAGE), which is consistent with the immunogen design targeting amino acids 1-100.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–100) of human catalase, suggesting the antibody epitope is located within this region; consider this when designing epitope competition or peptide blocking experiments.
• For Western blotting (WB) applications, a single band at approximately 60 kDa is anticipated in reducing conditions; validate in the relevant sample system (e.g., human, mouse, or rat cell lysates).
• In immunohistochemistry (IHC-P), peroxisomal staining may be optimized with antigen retrieval; liver and kidney tissues, rich in peroxisomes, serve as positive controls.
• For ELISA, the antibody’s peptide immunogen design allows potential use in capture or detection formats; consider pairwise testing with other catalase clones if developing a sandwich assay.
• Given cross-reactivity with mouse and rat, this antibody can be used for translational studies in rodent models of oxidative stress.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw-material sourcing, supporting diagnostic manufacturers and researchers from concept to clinic. For oxidative stress and peroxisomal biology studies, we supply catalase antibodies alongside a broad portfolio of validated antibody pairs and ancillary reagents. Our one-stop service extends to bulk reagent supply and custom sourcing of rare-target materials, streamlining your development workflow.