Anti-CAST Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P20810

Core Product Specifications and Parameters

Biological Background: CAST Function and Localization

• CAST encodes calpastatin, an endogenous specific inhibitor of calcium-dependent cysteine proteases (calpains), regulating intracellular proteolysis.
• Multiple isoforms are generated through alternative splicing, contributing to tissue-specific modulation of calpain activity.
• Calpastatin plays a key role in muscle protein degradation and postmortem meat tenderization by limiting calpain-mediated proteolysis.
• UniProt keywords indicate post-translational modifications including phosphorylation, acetylation, and ubiquitination (Ubl conjugation), which may influence its activity and stability.
• The protein contains conserved repeat domains essential for its inhibitory interaction with calpains.
• Mutations in CAST have been linked to palmoplantar keratoderma, suggesting a role in maintaining skin integrity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-350 of human CAST (NP_001177371.1), targeting the N-terminal region; consider potential isoform-specific detection.
• For Western blot, expect a band around 77 kDa; multiple bands may appear due to alternative splicing and post-translational modifications.
• In immunohistochemistry (IHC-P), validate staining specificity using appropriate tissue controls and antigen retrieval conditions.
• For ELISA, the antibody can be used to detect human CAST; optimization of coating and detection parameters is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, including validated antibodies, optimized monoclonal/polyclonal reagents, and bulk ancillary supplies. This anti-CAST antibody supports calpain-related research and muscle biology applications. Leverage our global supply network for rare target sourcing and comprehensive technical consulting to accelerate your projects from concept to clinic.