Anti-CASQ2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O14958

Core Product Specifications and Parameters

Biological Background: CASQ2 Function and Localization

• CASQ2 encodes calsequestrin-2, also known as the cardiac muscle isoform of calsequestrin (PDIB2), a high-capacity calcium-binding protein located in the sarcoplasmic reticulum lumen.
• Acts as an internal calcium store in muscle, binding approximately 60 Ca²⁺ ions per molecule via clusters of acidic residues on the protein surface, particularly at subunit interfaces.
• Regulates the release of lumenal Ca²⁺ through the cardiac ryanodine receptor (RYR2), thereby playing a critical role in excitation-contraction coupling and triggering muscle contraction.
• Essential for normal cardiac function, contributing to the regulation of heart rate and rhythm.
• Undergoes post-translational modifications including glycosylation and phosphorylation (UniProt keywords: Glycoprotein, Phosphoprotein).
• Alternatively spliced transcript variants exist, and mutations in CASQ2 are associated with disease variants (UniProt keywords: Alternative splicing, Disease variant).
• The protein has a molecular weight of approximately 46.4 kDa (calculated from sequence), consistent with the observed 46 kDa band.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 20-399 of human CASQ2, corresponding to the mature protein after signal peptide cleavage; this design enhances the likelihood of recognizing the native protein.
• For Western blot (WB), consider using a positive control such as human cardiac tissue lysate and verify a specific band around 46 kDa; optimize antibody dilution and incubation conditions for your specific sample.
• For immunohistochemistry on paraffin sections (IHC-P), ensure appropriate antigen retrieval and include proper controls to distinguish specific staining.
• For ELISA applications, titrate the antibody to determine the optimal coating or detection concentration; the antibody can be used in combination with a matched pair antibody if available.
• Cross-reactivity with mouse and rat has been reported; validate the antibody in these species under your experimental conditions, as reactivity may vary with sample type and protocol.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research labs, and institutes with a comprehensive portfolio of IVD raw materials, including validated antibodies for cardiac biomarkers and calcium signaling targets. The CASQ2 polyclonal antibody is part of our cardiac research and diagnostics support, enabling the development of assays for calcium handling proteins. In addition to individual antibodies, we offer bulk ancillary reagents and custom sourcing services to streamline your workflow from concept to clinic.