Anti-Caspr2/CNTNAP2 Monoclonal Antibody for WB, ELISA - Q9UHC6

Core Product Specifications and Parameters

Biological Background: Caspr2/CNTNAP2 Function and Localization

• Contactin-associated protein-like 2 (Caspr2), encoded by the CNTNAP2 gene, is a cell recognition molecule belonging to the neurexin family.
• Required, together with CNTNAP1, for the radial and longitudinal organization of myelinated axons, contributing to the formation of distinct axo-glial domains critical for saltatory conduction.
• Demarcates the juxtaparanodal region of the node of Ranvier, facilitating proper clustering of ion channels and efficient nerve impulse propagation.
• Required for gap junction formation (Probable).
• Predominantly expressed in the nervous system; localizes to the cell membrane, axon projections, and paranodal septate junctions.
• Contains EGF-like domains, laminin G-like domains, and a transmembrane region; undergoes alternative splicing and glycosylation.
• Associated with neurological disorders; linked to autism spectrum disorder, epilepsy, intellectual disability, and chromosomal rearrangements.

Experimental Guidance and Technical Tips

• The immunogen covers the intracellular C-terminal region (amino acids 1232-1331) of human Caspr2; this epitope is suitable for WB detection under appropriate denaturing conditions.
• For WB, consider using reducing SDS-PAGE with an expected molecular weight of ~148 kDa; validate transfer efficiency and use a loading control for accurate quantification.
• For ELISA, test the antibody against recombinant or native Caspr2 in a direct or capture format; include appropriate positive and negative controls.
• Confirmed cross-reactivity with mouse and rat allows its use in rodent models; verify specificity in the relevant tissue or cell line with suitable negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research teams with premium IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. For your neuroscience and rare target investigations, we source high-affinity antibodies like this anti-Caspr2/CNTNAP2 clone, tailored for applications such as WB and ELISA, streamlining your path from concept to clinic.