Anti-Caspr1 Rabbit Monoclonal Antibody for WB and ELISA - P78357

Core Product Specifications and Parameters

Biological Background: Caspr1 Function and Localization

• Caspr1 (Contactin-associated protein 1), also known as Neurexin IV, Neurexin-4, p190, is encoded by the CNTNAP1 gene. It is a single-pass type I transmembrane protein with a molecular weight of approximately 156 kDa.
• Caspr1 is required, together with CNTNAP2, for the radial and longitudinal organization of myelinated axons. It plays a critical role in forming the paranodal septate junctions, which are essential for saltatory conduction of nerve impulses.
• In association with contactin, Caspr1 mediates signaling between axons and myelinating glial cells, contributing to the maintenance of axo-glial interactions.
• Subcellularly, Caspr1 localizes to the paranodal region of the axo-glial junction, a specialized membrane domain at the node of Ranvier in myelinated nerve fibers.
• Caspr1 is predominantly expressed in the brain, with weak expression detected in other tissues such as ovary, pancreas, colon, lung, heart, intestine, and testis.
• The protein contains several structural motifs including EGF-like domains, disulfide bonds, and an SH3-binding motif, and undergoes glycosylation and phosphorylation. It has been associated with neuropathy in disease contexts, although specific mutations are linked to neurological disorders.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 700-800 of human Caspr1 (UniProt P78357). This region lies in the intracellular domain, which may contribute to target specificity. Consider verifying epitope recognition using recombinant protein or cell-based models.
• For Western blotting, the predicted protein weight is 156 kDa. Use a low-percentage SDS-PAGE gel (e.g., 6-8%) and appropriate transfer conditions to ensure efficient detection of this high-molecular-weight protein.
• In ELISA applications, cross-reactivity is reported for human only. Validate the antibody's performance with the relevant sample type and consider using a capture/detection antibody pair if needed.
• As a rabbit monoclonal antibody, it offers batch-to-batch consistency suitable for long-term studies. Ensure proper storage conditions (shipped on ice bags) and avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-Caspr1 rabbit monoclonal antibody is an important tool for neurobiology research, particularly for studying myelination and paranodal junction organization. As your sourcing partner, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and can assist in sourcing rare target raw materials to support your assay development needs. Whether you require customized solutions or technical guidance, CamelBio is committed to bridging the gap between research and clinical application.