Anti-Caspase-9 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P55211

Core Product Specifications and Parameters

Biological Background: Caspase-9 Function and Localization

• Caspase-9, also known as apoptotic protease Mch-6 (MCH6), APAF-3, or ICE-LAP6, is a member of the cysteine-aspartic acid protease (caspase) family and is encoded by the CASP9 gene in humans.
• It functions as an initiator caspase in the intrinsic apoptosis pathway. Upon cytochrome c release and Apaf-1 apoptosome formation, caspase-9 is recruited and activated, subsequently cleaving and activating effector caspases-3 and -7. It also cleaves poly(ADP-ribose) polymerase (PARP) and promotes DNA damage-induced apoptosis in an ABL1-dependent manner. Moreover, caspase-9 proteolytically cleaves BIRC6 following DIABLO/SMAC-mediated inhibition of the BIRC6-caspase complex. Related references: PMID:36758105 PMID:36758106
• Alternative splicing of CASP9 produces a catalytically inactive isoform that acts as a dominant-negative inhibitor of caspase-9 signaling.
• Caspase-9 is expressed ubiquitously, with highest levels in the heart where it is specifically found in myocytes. Moderate expression is observed in liver, skeletal muscle, and pancreas.
• Post-translational modifications include phosphorylation and ubiquitin-like conjugation, as indicated by proteomics data, which regulate its activity, stability, and interaction with other proteins.
• The protein is synthesized as an inactive zymogen that requires proteolytic processing for activation. As a thiol protease, it uses a catalytic cysteine residue for hydrolysis, and it is classified under apoptosis-related hydrolases.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody corresponds to amino acids 139–416 of human caspase-9 (NP_001220.2). This region covers part of the large catalytic domain, potentially recognizing both full-length and cleaved forms. For Western blot, a band at approximately 46 kDa may be detected for the proform, with additional bands corresponding to cleaved fragments depending on sample treatment.
• The antibody is recommended for Western blot (WB), immunofluorescence/immunocytochemistry (IF/ICC), immunohistochemistry (IHC-P), and ELISA. Researchers should optimize dilutions and include appropriate positive and negative controls, especially when working across human, mouse, and rat samples.
• Given that caspase-9 is mainly a cytoplasmic protein that translocates to mitochondria during apoptosis, subcellular localization may vary, and fixation/permeabilization conditions for IF/ICC or IHC-P should be adapted accordingly.

CamelBio: Your One-Stop Sourcing Bridge

• As a comprehensive IVD raw-material partner, CamelBio supplies high-quality antibodies like this anti-caspase-9 polyclonal antibody for apoptosis research, supporting diagnostic developers and research institutes from target validation through assay design.
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• Contact CamelBio to explore how our optimized products and services can accelerate your diagnostic and research workflows.