Anti-Caspase-9 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P55211

Core Product Specifications and Parameters

Biological Background: Caspase-9 Function and Localization

• Caspase-9 is a cysteine protease of the caspase family that functions as an initiator caspase in the intrinsic apoptotic pathway.
• Upon binding to Apaf-1, caspase-9 becomes activated and subsequently cleaves and activates effector caspases, such as caspase-3 and caspase-7, to execute apoptosis.
• Caspase-9 promotes DNA damage-induced apoptosis in an ABL1/c-Abl-dependent manner and proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
• Caspase-9 cleaves BIRC6 following the inhibition of BIRC6-caspase binding by DIABLO/SMAC. Related references: PMID:36758105, PMID:36758106
• An alternatively spliced isoform, Caspase-9S, lacks catalytic activity and acts as a dominant-negative inhibitor of the full-length enzyme.
• Ubiquitously expressed with highest levels in the heart (specifically in myocytes), moderate in liver, skeletal muscle, and pancreas, and low in other tissues.
• Post-translational modifications include phosphorylation and ubiquitin conjugation, regulating its activity; synthesized as a zymogen requiring proteolytic processing.
• Keywords: Apoptosis, Hydrolase, Protease, Thiol protease, Phosphoprotein, Ubl conjugation, Zymogen, Alternative splicing, 3D-structure.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 139-416 of human caspase-9, which includes the large subunit; the antibody may detect both the full-length proform (~46 kDa) and processed forms. Include appropriate molecular weight markers.
• For Western blot, consider using heart or liver tissue lysates as positive controls, given the relatively high expression in these tissues.
• In immunohistochemistry, validate staining in heart or liver sections and optimize antigen retrieval conditions for formalin-fixed paraffin-embedded tissues.
• For immunofluorescence, caspase-9 localizes primarily to the cytoplasm; consider co-staining with mitochondrial markers to assess apoptotic activation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with one-stop access to validated IVD raw materials, including high-quality polyclonal antibodies like this anti-caspase-9 antibody, as well as antibody pairs, recombinant proteins, and ancillary reagents for apoptosis research. We streamline procurement from concept to clinic, assisting with rare-target sourcing and bulk-supply needs to accelerate your assay development.