Anti-Caspase-8 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q14790

Core Product Specifications and Parameters

Biological Background: Caspase-8 Function and Localization

• CASP8 encodes a thiol protease that serves as a molecular switch for apoptosis, necroptosis, and pyroptosis, and is essential for preventing tissue damage during embryonic development and adulthood. Related references: PMID:23516580, PMID:35338844, PMID:35446120
• In extrinsic apoptosis, CASP8 is recruited to death receptors (FAS/TNFRSF1A) via the adaptor FADD, forming the death-inducing signaling complex (DISC) where it undergoes proteolytic activation. Related references: PMID:8681376, PMID:8681377, PMID:9184224
• Activated CASP8 cleaves and activates downstream effector caspases (CASP3, CASP4, CASP6, CASP7, CASP9, CASP10) as well as BID, linking death receptor signaling to the mitochondrial apoptosis pathway. Related references: PMID:16916640, PMID:8962078, PMID:9006941
• CASP8 also acts as a negative regulator of necroptosis by cleaving RIPK1 at Asp-324, limiting TNF-induced necroptosis and inflammation. Related references: PMID:31827280, PMID:31827281
• In pyroptosis, CASP8 cleaves gasdermin-C and -D (GSDMC, GSDMD), generating pore-forming N-terminal fragments that trigger membrane permeabilization. Related references: PMID:32929201, PMID:34012073
• Subcellularly, CASP8 localizes to the cytoplasm, nucleus, and cell projections such as lamellipodia.
• Isoform 1, 5, and 7 are broadly expressed, with highest levels in peripheral blood leukocytes, spleen, thymus, and liver; alternatively spliced isoforms lacking catalytic activity can act as dominant-negative inhibitors of the caspase cascade. Related references: PMID:12010809

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 376–479) of human Caspase-8; both full-length and cleaved fragments may be detected in Western blot—validate band patterns in your experimental system.
• For Western blot, expect a band near 55 kDa for the proenzyme; additional bands representing active cleaved fragments may appear upon apoptosis induction.
• In immunohistochemistry (IHC-P), consider antigen retrieval with citrate or Tris-EDTA buffer and optimize blocking conditions for your tissue.
• For immunofluorescence (IF/ICC), adjust fixation and permeabilization to balance antigen accessibility and morphological preservation.
• The antibody is predicted to cross-react with mouse and rat based on manufacturer data; perform sequence alignment to confirm epitope conservation if working with other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD assay development by providing a comprehensive portfolio of high-quality raw materials, including this anti-Caspase-8 polyclonal antibody for apoptosis and cell death research. As your one-stop sourcing partner, we facilitate access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material procurement—supporting your projects from concept to clinic.