Anti-Caspase-8 Rabbit Monoclonal Antibody for WB, IP, IF/ICC, ELISA - O89110

Core Product Specifications and Parameters

Biological Background: Caspase-8 Function and Localization

• Caspase-8 is a thiol protease that serves as a molecular switch for programmed cell death, integrating signals for apoptosis, necroptosis, and pyroptosis to prevent tissue damage during development and adulthood. Related references: PMID:12065591 PMID:18455983 PMID:30361383
• It acts as the initiator caspase in the extrinsic apoptosis pathway, recruited to death receptors (FAS/CD95, TNFRSF1A) via FADD to form the death-inducing signaling complex (DISC), where it is proteolytically activated and subsequently cleaves downstream effector caspases (CASP3, CASP6, CASP7) and BID to trigger mitochondrial cytochrome C release. Related references: PMID:24813849 PMID:24813850 PMID:9654089 PMID:9727491 PMID:9727492
• Caspase-8 negatively regulates necroptosis by cleaving RIPK1 at Asp-325, a critical event that prevents RIPK1 auto-phosphorylation and limits TNF-induced cell death and inflammation. Related references: PMID:31511692
• Under specific conditions (e.g., TAK1 inhibition), Caspase-8 can initiate pyroptosis by cleaving and activating gasdermin-C (GSDMC) and gasdermin-D (GSDMD), leading to pore formation and lytic cell death. Related references: PMID:30361383 PMID:30381458
• It also functions in innate immunity by cleaving and inactivating the negative regulator N4BP1 downstream of TLR3 and TLR4, thus promoting cytokine production. Related references: PMID:32971525
• Caspase-8 is predominantly cytoplasmic but can also localize to the nucleus. It is widely expressed, with highest levels in spleen, thymus, lung, liver, and kidney, and lower levels in heart, brain, testis, and skeletal muscle.
• The protein is subject to acetylation and phosphorylation, and it is synthesized as an inactive zymogen requiring dimerization and cleavage for full activity. It belongs to the peptidase C14A family of cysteine-dependent aspartate-directed proteases.

Experimental Guidance and Technical Tips

• The immunogen spans residues 1-218 of mouse Caspase-8, encompassing the N-terminal prodomain. Consider this region when designing detection strategies, as antibodies may recognize both full-length and partially processed forms.
• For Western blot, a band around 55 kDa is expected, but smaller cleavage fragments may appear upon apoptosis induction. Validate specificity using appropriate positive and negative controls in your cell system.
• Immunoprecipitation and immunofluorescence/ICC are compatible with this rabbit monoclonal antibody. Optimize fixation and permeabilization steps for intracellular targets, and include isotype controls.
• In ELISA, use this antibody as a capture or detection reagent in sandwich formats. Confirm performance with recombinant protein standards to establish linearity and sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a trusted one-stop partner for IVD raw-material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your assay development from concept to clinic. For apoptosis and cell death research, we provide high-quality reagents targeting critical molecules like Caspase-8, along with rare-target sourcing capabilities. Contact us to streamline your procurement and accelerate your diagnostic innovation.