Anti-Caspase-7 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P55210

Core Product Specifications and Parameters

Biological Background: Caspase-7 Function and Localization

• Caspase-7 (also known as MCH3, CMH-1, ICE-LAP3) is a cysteine-aspartic acid protease encoded by the CASP7 gene; an alternative splicing isoform lacking enzymatic activity has been reported.
• As an effector caspase in the execution phase of apoptosis, cleaved and activated by initiator caspases (CASP8, CASP9, CASP10), it mediates cleavage of substrates such as CLSPN, PARP1, PTGES3, and YY1 to promote programmed cell death. Related references: PMID:10497198 PMID:16123041 PMID:16374543
• Exhibits a marked preference for DEVD consensus sequences but can accommodate non-canonical motifs, highlighting substrate versatility. Related references: PMID:12824163 PMID:15314233 PMID:17697120
• Regulates inflammatory responses and pyroptosis: upon activation by CASP1 in intestinal epithelial cells, cleaves and activates SMPD1 to generate ceramides that repair membranes and counter gasdermin-D pores.
• Also participates in granzyme-mediated cell death in hepatocytes, where it is activated by GZMB and secreted to cleave SMPD1, balancing perforin pore formation.
• Subcellular localization includes cytoplasm, cytosol, nucleus, and secretion into the extracellular space, consistent with its diverse roles in proteolysis and membrane repair.
• Highly expressed in lung, skeletal muscle, liver, kidney, spleen, and heart; moderate in testis; absent in brain.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination; it is synthesized as an inactive zymogen requiring proteolytic processing for activation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region of Caspase-7 (aa 150–250), which may detect both pro- and cleaved forms; validate in relevant sample systems to confirm specificity.
• For WB, the predicted molecular weight is ~34 kDa; a band shift may be observed upon activation, so consider including induced and uninduced controls.
• IHC-P and IF/ICC applications may require optimization for antigen retrieval; testing both paraffin-embedded and frozen sections is recommended.
• ELISA applications can be developed into sandwich assays when paired with a suitable capture antibody; perform cross-reactivity checks against related caspase family members.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, providing diagnostic manufacturers, laboratories, and research institutes with validated antibodies, antigen pairs, and ancillary reagents. Our Caspase-7 rabbit polyclonal antibody supports apoptosis, pyroptosis, and cell death research, and is available in bulk quantities with batch-to-batch consistency. CamelBio also offers customized sourcing for rare targets, technical consulting, and supply chain solutions to accelerate your diagnostic development from concept to clinic.