Anti-Caspase-6 Rabbit Polyclonal Antibody for WB, ELISA - P55212

Core Product Specifications and Parameters

Biological Background: Caspase-6 Function and Localization

• Caspase-6 is a cysteine protease that plays essential roles in apoptosis, axonal degeneration, development, and innate immunity. Related references: PMID:19133298, PMID:22858542, PMID:27032039
• It acts as a non-canonical executioner caspase during apoptosis, localizing to the nucleus where it cleaves nuclear structural proteins NUMA1 and lamin A/LMNA, inducing nuclear shrinkage and fragmentation. Related references: PMID:11953316, PMID:17401638, PMID:8663580
• Cleavage of lamin A/LMNA is required for chromatin condensation and nuclear disassembly during apoptotic execution. Related references: PMID:11953316
• Caspase-6 regulates liver damage by promoting hepatocyte apoptosis; when not phosphorylated by AMPK, it cleaves BID, leading to cytochrome c release, contributing to nonalcoholic steatohepatitis. Related references: PMID:32029622
• It is involved in intrinsic apoptosis by mediating cleavage of RIPK1 and cleaves transcription factors such as NF-kappa-B and CREBBP. Related references: PMID:22858542, PMID:10559921, PMID:14657026
• Beyond apoptosis, Caspase-6 participates in PANoptosis (pyroptosis, apoptosis, necroptosis) independently of its protease activity, serving as a scaffold for ZBP1-mediated cell death during viral infection. Related references: PMID:32298652
• Plays an essential role in axon degeneration during developmental pruning (By similarity).
• During microbial infection, it proteolytically cleaves coronavirus N protein, including MERS-CoV, SARS-CoV, and SARS-CoV-2, modulating IFN signaling to enhance viral replication. Related references: PMID:18155731, PMID:35922005

Experimental Guidance and Technical Tips

• The immunogen corresponds to a sequence within the mature caspase-6 subunit (aa 194-293), which may favor detection of both the pro-form and active cleaved fragments. Consider verifying specificity in relevant cell lysates.
• For Western blot, a band around 33kDa (pro-form) and possibly lower molecular weight bands (~18kDa and ~11kDa active subunits) may be observed depending on apoptosis induction.
• Validate antibody performance in your assay using positive and negative controls, e.g., untreated vs. apoptotic cells.
• For ELISA, pairing with a suitable capture antibody may improve sensitivity. Titration is recommended.
• Cross-reactivity with mouse Caspase-6 has been reported; ensure sequence homology if extending to other species.
• Store as recommended on ice bag shipping; long-term storage at -20°C or -80°C in aliquots to avoid freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials, including validated antibodies, antigens, and ancillary reagents. For those investigating apoptosis, neurodegeneration, or antiviral mechanisms, Caspase-6 antibodies are available alongside paired reagents to accelerate assay development. Explore our full catalog to source everything from custom monoclonal antibodies to rare target raw materials, backed by technical consulting from concept to clinic.