Anti-Caspase-6 Rabbit Monoclonal Antibody for WB - P55212

Core Product Specifications and Parameters

Biological Background: Caspase-6 Function and Localization

• Caspase-6 (MCH2) is a cysteine protease belonging to the caspase family, acting as a non-canonical executioner caspase in apoptosis.
• It localizes to the nucleus during apoptosis and cleaves nuclear structural proteins NUMA1 and lamin A/C to induce nuclear shrinkage and fragmentation. Related references: PMID:11953316, PMID:17401638, PMID:8663580
• Under conditions of reduced AMPK phosphorylation, it cleaves BID to trigger cytochrome c release, contributing to hepatocyte apoptosis and nonalcoholic steatohepatitis. Related references: PMID:32029622
• Cleaves key transcription factors such as NF-kappa-B and CREBBP, modulating inflammatory and survival pathways. Related references: PMID:10559921, PMID:14657026
• Serves as a scaffold in ZBP1-mediated PANoptosis, a programmed cell death mechanism integrating pyroptosis, apoptosis, and necroptosis, independently of its proteolytic activity. Related references: PMID:32298652
• Plays an essential role in axon degeneration and pruning during neurogenesis.
• Cleaves the nucleocapsid (N) protein of coronaviruses, including SARS-CoV-2, to regulate viral replication and host interferon responses. Related references: PMID:18155731, PMID:35922005
• Predominantly found in the cytoplasm and nucleus.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 45-162 of human Caspase-6, a region that may help detect both pro- and cleaved forms.
• For WB, expect bands around 33 kDa (proform) and potential cleaved fragments; validate in relevant cell lines or tissues undergoing apoptosis.
• For IF/ICC, expect nuclear and cytoplasmic staining; use appropriate permeabilization methods and validate in your sample system.
• For ELISA, consider pairing with a detection antibody recognizing a different epitope; the antibody can capture or detect target.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing solution for IVD raw materials, enabling diagnostic manufacturers to access validated antibody pairs, monoclonal antibodies, and bulk ancillary reagents for apoptosis and cell death research. For Caspase-6 studies, we offer high-quality reagents and technical services to support your projects from concept to clinic. Contact us to streamline your supply chain and accelerate diagnostic development.