Anti-Caspase-6 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P55212

Core Product Specifications and Parameters

Biological Background: Caspase-6 Function and Localization

• Caspase-6 is a cysteine protease expressed as a zymogen that undergoes autocatalytic cleavage for activation. It is subject to alternative splicing, palmitoylation, and phosphorylation, which regulate its activity and localization.
• It plays essential roles in programmed cell death (apoptosis), axonal degeneration, development, and innate immunity (PubMed:19133298, PubMed:22858542, PubMed:27032039).
  Related references: PMID:19133298, PMID:22858542, PMID:27032039
• As a non-canonical executioner caspase, it localizes to the nucleus and cleaves NUMA1 and lamin A/LMNA, inducing nuclear shrinkage and fragmentation. Lamin A cleavage is required for chromatin condensation and nuclear disassembly during apoptosis (PubMed:11953316, PubMed:17401638, PubMed:8663580).
  Related references: PMID:11953316, PMID:17401638, PMID:8663580
• Caspase-6 cleaves transcription factors NF-kappa-B and CREBBP, as well as phospholipid scramblase proteins XKR4 and XKR9, influencing gene expression and membrane dynamics (PubMed:10559921, PubMed:14657026).
  Related references: PMID:10559921, PMID:14657026
• In PANoptosis, Caspase-6 acts as a molecular scaffold independent of its protease activity, interacting with RIPK3 and ZBP1 to trigger pyroptosis, apoptosis, and necroptosis (PubMed:32298652).
  Related references: PMID:32298652
• In liver, it promotes hepatocyte apoptosis by cleaving BID under conditions of AMPK dysregulation, contributing to nonalcoholic steatohepatitis (PubMed:32029622).
  Related references: PMID:32029622
• (Microbial infection) It proteolytically cleaves coronavirus N proteins (MERS-CoV, SARS-CoV, SARS-CoV-2), modulating viral replication by inhibiting IRF3 nuclear translocation and suppressing IFN signaling (PubMed:18155731, PubMed:35922005).
  Related references: PMID:18155731, PMID:35922005
• Subcellularly, Caspase-6 is found in both cytoplasm and nucleus, with nuclear localization being critical for its executioner function in apoptosis.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 150-250 of human Caspase-6. This central region is conserved, supporting cross-reactivity with mouse and rat.
• For Western blot, anticipate a band near 33 kDa. Use reducing conditions and validate expression in your cell or tissue lysate of interest; consider including positive controls like apoptotic cells.
• In IHC-P, nuclear and cytoplasmic staining may be observed. Antigen retrieval and antibody dilution should be optimized empirically.
• For ELISA, pairing with a detection antibody against a non-overlapping epitope is recommended to improve assay sensitivity.
• The antibody’s reported cross-reactivity with mouse and rat enables translational studies; always verify specificity in the relevant model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw-material partner, bridging the gap from concept to clinic with reliable antibodies, matched antibody pairs, and ancillary reagents. This anti-Caspase-6 monoclonal antibody supports research in apoptosis, neurodegeneration, and infectious disease, and our team can assist in sourcing complementary products such as recombinant Caspase-6 proteins, substrate peptides, and custom assay development materials to accelerate your diagnostic pipeline.