Anti-Caspase-6 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P55212

Core Product Specifications and Parameters

Biological Background: Caspase-6 Function and Localization

• Caspase-6 (Mch-2) is a cysteine‑aspartic acid protease encoded by the CASP6 gene (GeneID 839), with a molecular weight of ~33 kDa.
• It acts as a non‑canonical executioner caspase during apoptosis: localizing in the nucleus, it cleaves the nuclear structural proteins lamin A (LMNA) and NUMA1, inducing nuclear shrinkage and fragmentation. Lamin A cleavage is specifically required for chromatin condensation and nuclear disassembly. Related references: PMID:11953316, PMID:17401638, PMID:8663580
• Upon loss of AMPK‑mediated phosphorylation, caspase‑6 cleaves BID, triggering cytochrome c release and promoting hepatocyte apoptosis in nonalcoholic steatohepatitis. Related references: PMID:32029622
• In the intrinsic apoptosis pathway, caspase‑6 mediates cleavage of RIPK1, contributing to the apoptotic signal. Related references: PMID:22858542
• It cleaves transcription factors NF‑kappa‑B and CREBBP, thereby modulating inflammatory and transcriptional programs during cell death. Related references: PMID:10559921, PMID:14657026
• Independent of its protease activity, caspase‑6 serves as a scaffold facilitating ZBP1‑mediated PANoptosis (pyroptosis, apoptosis, necroptosis) by enhancing the interaction between RIPK3 and ZBP1, leading to inflammasome activation. Related references: PMID:32298652
• Caspase‑6 also plays an antiviral role by proteolytically cleaving the N protein of coronaviruses (e.g., MERS‑CoV, SARS‑CoV, SARS‑CoV‑2), which suppresses type I interferon signaling and enhances viral replication. Related references: PMID:18155731, PMID:35922005
• The protein resides in both the cytoplasm and nucleus, and its activity is regulated by proteolytic processing, phosphorylation, and palmitoylation (lipoprotein).

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within amino acids 100‑200 of human caspase‑6 (P55212), which lies in the p20 large subunit; the antibody may recognize both the ~33 kDa proform and the p20 cleavage fragment.
• For Western blotting, the predicted band for full‑length caspase‑6 is ~33 kDa; upon activation, smaller fragments (~20 kDa and ~10 kDa) may appear. Validate detection in apoptotic vs. non‑apoptotic lysates.
• In immunofluorescence/ICC, expect dual cytoplasmic‑nuclear staining; translocation to the nucleus can be a marker of apoptosis induction. Optimize fixation/permeabilization for reliable detection.
• The antibody is suitable for ELISA; consider pairing with a second antibody recognizing a distant epitope for sandwich assay development.
• Cross‑reactivity has been confirmed for human and mouse; for other species, sequence alignment of the immunogen region and experimental validation are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials, including this anti‑Caspase‑6 monoclonal antibody, to support research and diagnostic development in apoptosis, neurodegeneration, and innate immunity. Our portfolio includes validated antibody pairs, optimized monoclonals and polyclonals, and bulk ancillary reagents, enabling seamless assay assembly from early‑stage research to clinical application. For rare‑target sourcing or customized antibody development, CamelBio’s technical team is ready to assist you in bridging the gap between concept and clinic.