Anti-Caspase-6 Monoclonal Antibody for WB, ELISA - P55212

Core Product Specifications and Parameters

Biological Background: Caspase-6 Function and Localization

• Caspase-6 is a cysteine protease that plays essential roles in programmed cell death, axonal degeneration, development, and innate immunity. Related references: PMID:19133298, PMID:22858542, PMID:27032039
• Acts as a non-canonical executioner caspase during apoptosis, localizing in the nucleus and cleaving nuclear structural protein NUMA1 and lamin A/LMNA to induce nuclear shrinkage and fragmentation. Related references: PMID:11953316, PMID:17401638, PMID:8663580
• Lamin A/LMNA cleavage is required for chromatin condensation and nuclear disassembly during apoptotic execution. Related references: PMID:11953316
• Regulates liver damage by promoting hepatocyte apoptosis: in absence of phosphorylation by AMPK, cleaves BID, leading to cytochrome c release. Related references: PMID:32029622
• Involved in intrinsic apoptosis by mediating cleavage of RIPK1. Related references: PMID:22858542
• Plays an essential role in defense against viruses by acting as a central mediator of ZBP1-mediated PANoptosis, independently of its protease activity. Related references: PMID:32298652
• (Microbial infection) Proteolytically cleaves the N protein of coronaviruses such as MERS-CoV, SARS-CoV, and SARS-CoV-2, modulating IFN signaling and enhancing viral replication. Related references: PMID:18155731, PMID:35922005
• Subcellular localization includes both cytoplasm and nucleus.
• Expressed as a zymogen and activated by autocatalytic cleavage; also subject to phosphorylation and palmitoylation.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide derived from human Caspase-6, suggesting specificity for the target protein in denaturing and native conditions.
• For Western blot, the predicted molecular weight of Caspase-6 is ~33 kDa, but due to cleavage, multiple bands may be observed; validation in the relevant sample system is recommended.
• For ELISA, consider pairing with a capture antibody if developing a sandwich assay; this antibody may be suitable as a detection reagent.
• Cross-reactivity is reported for human, mouse, and rat; however, optimal dilutions and conditions should be determined experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop provider of IVD raw materials, technical services, and consulting, supporting diagnostic manufacturers from research to clinic. This anti-Caspase-6 antibody is part of our extensive portfolio, suitable for apoptosis, neurobiology, and innate immunity research applications. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing solutions for rare or challenging targets to accelerate your assay development.