Anti-Caspase-4 Rabbit pAb for WB, IHC-P, ELISA - P49662

Core Product Specifications and Parameters

Biological Background: Caspase-4 Function and Localization

• Caspase-4 (also known as TX, Mih1, ICH-2) is an inflammatory caspase and a member of the cysteine-aspartic acid protease (caspase) family.
• It serves as the effector of the non-canonical inflammasome, directly binding lipopolysaccharide (LPS) to induce pyroptosis.
  Related references: PMID:25119034 PMID:25121752 PMID:26375003 PMID:32109412
• Caspase-4 cleaves gasdermin D (GSDMD) to trigger pore formation and pyroptotic cell death, and processes interleukin-18 (IL18) but not IL1B directly.
  Related references: PMID:15326478 PMID:23516580 PMID:26375003 PMID:37993712 PMID:37993714
• It indirectly activates the NLRP3 and NLRP6 inflammasomes, promoting secretion of mature cytokines like IL1A and HMGB1.
  Related references: PMID:23516580 PMID:26375003 PMID:32109412
• Caspase-4 localizes to the cytoplasm, endoplasmic reticulum membrane, mitochondria, and is also secreted; it is a component of the inflammasome.
• The protein is widely expressed, including in keratinocytes and intestinal epithelial cells, but not detected in brain.
• Key post-translational modifications include acetylation and phosphorylation; it is synthesized as a zymogen and undergoes autoproteolytic processing.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide (a.a. 81-270 of human Caspase-4); thus, the antibody may recognize both full-length and cleaved fragments. For WB, consider using reducing conditions and probing around 43 kDa.
• For IHC-P, antigen retrieval conditions may need optimization; validate in human tissue sections known to express caspase-4, such as colonic epithelium.
• ELISA performance can be assessed by direct coating of peptide or recombinant protein; include appropriate controls for specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents, streamlining your assay development from concept to clinic. This Caspase-4 antibody can be integrated into research on inflammatory disorders, pyroptosis, and innate immunity. For rare targets or customized sourcing needs, CamelBio provides dedicated technical support and supply chain solutions.