Anti-Caspase-3 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P42574

Core Product Specifications and Parameters

Biological Background: Caspase-3 Function and Localization

• Caspase-3 (CPP32, Apopain, Yama, SCA-1) is a major effector caspase that executes apoptosis by cleaving multiple cellular substrates following activation by initiator caspases.
  Related references: PMID:18723680, PMID:20566630, PMID:23650375

• It proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a specific Asp-Gly bond, a hallmark of apoptosis.
  Related references: PMID:10497198, PMID:16374543, PMID:7596430

• Caspase-3 also activates other caspases (CASP6, CASP7, CASP9) and inactivates interleukin-18 (IL18), amplifying the apoptotic cascade.
  Related references: PMID:7596430, PMID:37993714, PMID:9334240

• It mediates pyroptosis by cleaving gasdermin-E (GSDME) and inhibits type I interferon production during viral infection by targeting CGAS, IRF3, and MAVS.
  Related references: PMID:30878284, PMID:35338844, PMID:35446120

• Caspase-3 cleaves XRCC4 and phospholipid scramblase proteins (XKR4, XKR8, XKR9) to promote phosphatidylserine exposure on apoptotic cells.
  Related references: PMID:23845944, PMID:33725486

• Caspase-3 is implicated in the cleavage of huntingtin and modulates cell adhesion in sympathetic neurons via RET cleavage.
  Related references: PMID:8696339, PMID:21357690

• It is localized in the cytoplasm, with high expression in lung, spleen, heart, liver, kidney, and immune cells; moderate levels in brain and skeletal muscle; and low in testis. Post-translational modifications include acetylation, phosphorylation, and S-nitrosylation.

Experimental Guidance and Technical Tips

• For Western blot, based on the immunogen design (amino acids 176-277, corresponding to the mature large subunit region), the antibody may detect both the 32kDa proenzyme and the 17kDa cleaved fragment. Consider using apoptosis-induced lysates as a positive control.
• For immunohistochemistry on paraffin-embedded tissues, optimal antigen retrieval conditions and antibody dilution should be determined empirically; endogenous peroxidase blocking is recommended.
• The antibody has shown cross-reactivity with mouse and rat Caspase-3, making it suitable for multi-species apoptosis studies. Validate in your specific sample system to confirm reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD raw-material procurement by providing validated antibodies, antigen pairs, and bulk reagents for diagnostic development. This anti-Caspase-3 antibody is part of a comprehensive portfolio supporting apoptosis research and assay design. Partner with us for reliable sourcing and expert technical guidance from concept to commercialization.