Anti-Caspase-3 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P42574

Core Product Specifications and Parameters

Biological Background: Caspase-3 Function and Localization

• Caspase-3 is a thiol protease that acts as a major effector caspase in the execution phase of apoptosis. Upon activation by initiator caspases (CASP8, CASP9, and/or CASP10), it mediates apoptosis by catalyzing the cleavage of numerous protein substrates. Related references: PMID:18723680 PMID:20566630 PMID:23650375
• A hallmark apoptotic event is the proteolytic cleavage of poly(ADP-ribose) polymerase PARP1 at the Asp216-Gly217 bond. Caspase-3 also cleaves and activates downstream caspases such as CASP6, CASP7, and CASP9, and inactivates interleukin-18 (IL18). Related references: PMID:10497198 PMID:16374543 PMID:7596430 PMID:37993714 PMID:9334240
• Beyond apoptosis, caspase-3 is involved in pyroptosis by mediating cleavage and activation of gasdermin-E (GSDME), linking it to inflammatory cell death pathways. Related references: PMID:35338844 PMID:35446120
• Caspase-3 contributes to non-apoptotic roles, including axon pruning during brain development, cell adhesion regulation via RET and desmosomal protein cleavage (e.g., DSG2, JUP), and cleavage of huntingtin. Related references: PMID:8696339 PMID:21357690 PMID:17559062
• Under oxidative stress, caspase-3 cleaves and inhibits serine/threonine-protein kinase AKT1, and during viral infection, it cleaves antiviral proteins CGAS, IRF3, and MAVS to prevent cytokine overproduction. Related references: PMID:23152800 PMID:30878284
• The protein localizes predominantly in the cytoplasm and is expressed at high levels in lung, spleen, heart, liver, and kidney, with moderate levels in brain and skeletal muscle, and low levels in testis. It is most abundant in immune system cells.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 55–160 of human Caspase-3, spanning part of the pro-domain and large subunit; consider that this antibody may recognize both the inactive pro-form and the cleaved active form.
• For Western blot (WB), the predicted molecular weight is approximately 32 kDa, but cleavage fragments (e.g., ~17 kDa and ~12 kDa) may appear depending on activation state and sample preparation.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), validate staining in known positive tissues (e.g., spleen, thymus) and include appropriate controls to confirm specificity.
• Given the high sequence identity across human, mouse, and rat, cross-reactivity is expected; however, optimal dilution and incubation conditions should be determined empirically for each species and application.
• Store as recommended (ice bag shipping) and avoid repeated freeze-thaw cycles to maintain antibody stability and performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated polyclonal antibodies like this anti-Caspase-3 antibody to support apoptosis and cell death research. With a portfolio spanning optimized antibody pairs, bulk ancillary reagents, and access to rare-target raw materials, CamelBio empowers diagnostic manufacturers and laboratories to accelerate development from concept to clinical application. Explore our one-stop supply solutions tailored to your workflow needs.