Anti-Caspase-3 Monoclonal Antibody for WB, ELISA - P42574

Core Product Specifications and Parameters

Biological Background: Caspase-3 Function and Localization

• Caspase-3 (also known as apopain, CPP32, Yama, and SCA-1) is a thiol protease that functions as a primary executioner caspase in the apoptotic cascade.
• Mediates the execution phase of apoptosis by cleaving a wide range of cellular substrates, including the hallmark cleavage of poly(ADP-ribose) polymerase PARP1 at Asp216-Gly217, and activating downstream caspases-6, -7, and -9 to amplify the proteolytic cascade. Related references: PMID:18723680 PMID:20566630 PMID:23650375 PMID:10497198 PMID:16374543 PMID:7596430
• Cleaves interleukin-18 (IL18), huntingtin, RET, desmosomal cadherins DSG2 and JUP, and AKT1, and inactivates antiviral innate immune proteins (CGAS, IRF3, MAVS) during virus-induced apoptosis; also cleaves gasdermin-E (GSDME) to trigger pyroptosis. Related references: PMID:37993714 PMID:9334240 PMID:8696339 PMID:21357690 PMID:17559062 PMID:23152800 PMID:30878284 PMID:35338844 PMID:35446120
• Promotes phosphatidylserine exposure on the apoptotic cell surface by cleaving scramblases XKR4, XKR8, and XKR9, and ensures apoptosis progression by cleaving BIRC6 after DIABLO/SMAC-mediated inhibition. Related references: PMID:23845944 PMID:33725486 PMID:36758104 PMID:36758106
• Localizes predominantly to the cytoplasm.
• Highly expressed in lung, spleen, heart, liver, and kidney, with moderate levels in brain and skeletal muscle and low levels in testis; expression is particularly high in immune system cells.
• Subject to multiple post-translational modifications, including acetylation, phosphorylation, S-nitrosylation, and ubiquitination, which regulate its activity and stability.

Experimental Guidance and Technical Tips

• For Western blotting, the antibody may detect both the full-length pro-caspase-3 (~32 kDa) and the cleaved large fragment (~17-19 kDa) due to the immunogen sequence (aa 55-160) located within the p17 large subunit. Consider using apoptotic cell lysates (e.g., Jurkat treated with staurosporine) as positive controls.
• In ELISA applications, the recombinant fusion protein corresponding to the immunogen region can be employed as a coating antigen. Validate antibody performance with appropriate standards and sample matrices.
• The antibody cross-reacts with mouse caspase-3, allowing its use in mouse-derived samples. Titration and optimization under your specific experimental conditions are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing solution for IVD raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare-target raw-material sourcing. This anti-caspase-3 monoclonal antibody supports apoptosis research and diagnostic assay development; our team is ready to assist with the full supply chain, helping you accelerate projects from concept to clinic.