Anti-Caspase-2 Rabbit Polyclonal Antibody for WB and ELISA - P42575

Core Product Specifications and Parameters

Biological Background: Caspase-2 Function and Localization

• Caspase-2 (official symbol CASP2) is a thiol protease of the caspase family, also known as ICH-1 and NEDD-2. The CASP2 gene undergoes alternative splicing, producing multiple isoforms.
• It acts as a key regulator of the apoptotic cascade, executing cell death through activation of downstream caspases or inactivation of survival proteins (PubMed:11156409, PubMed:15073321, PubMed:8087842). Caspase-2 associates with PIDD1 and CRADD to form the PIDDosome complex, which triggers apoptosis in response to genotoxic stress (PubMed:15073321).
  Related references: PMID:11156409 PMID:15073321 PMID:8087842
• Caspase-2 exhibits a dual role in apoptosis, functioning as both a positive and negative regulator, and may act as an endogenous inhibitor that suppresses caspase activation and cell death (PubMed:15073321).
  Related references: PMID:15073321
• The protein is expressed at higher levels in embryonic lung, liver, and kidney compared to heart and brain. In adult tissues, elevated expression is observed in the placenta, lung, kidney, and pancreas.
• Caspase-2 undergoes key post-translational modifications, including phosphorylation and acetylation, and is initially synthesized as an inactive zymogen that requires proteolytic processing for activation.
• Mutations in the CASP2 gene are associated with disease variants, including intellectual disability and lissencephaly, highlighting its critical role in neuronal development.

Experimental Guidance and Technical Tips

• For Western blot (WB), validate the antibody using lysates from caspase-2 positive cell lines or tissues, and consider including a negative control with caspase-2 knockdown or knockout to confirm specificity. The predicted molecular weight is approximately 51 kDa; use appropriate protein markers and consider adding protease inhibitors to the lysis buffer to prevent caspase-2 degradation during sample preparation.
• In ELISA applications, optimize coating conditions using the full-length recombinant protein or the immunogen fragment, and ensure proper blocking to minimize non-specific binding.
• Given the reported cross-reactivity with mouse and rat, verify the antibody’s reactivity in these species by testing relevant tissue lysates or recombinant proteins.
• As caspase-2 is involved in apoptosis induction, treat cells with known apoptotic inducers (e.g., DNA-damaging agents) to enrich for the activated caspase-2 forms and enhance detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to supporting diagnostic manufacturers and researchers by providing one-stop access to high-quality IVD raw materials and technical services. Our portfolio includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, recombinant proteins, and ancillary reagents for apoptosis research targets such as caspase-2. With a focus on streamlining procurement and offering expert consultation, CamelBio helps you efficiently source reliable raw materials to advance your assay development from concept to commercialization.