Anti-Caspase-2 Monoclonal Antibody for WB, ELISA - P42575

Core Product Specifications and Parameters

Biological Background: Caspase-2 Function and Localization

• Caspase-2 (also known as ICH-1, NEDD-2) is a cysteine protease belonging to the caspase family, expressed as a 51kDa proenzyme in humans.
• It functions as a key regulator of the apoptotic caspase cascade, capable of both promoting and inhibiting apoptosis depending on cellular context (PubMed:11156409, PubMed:15073321, PubMed:8087842). Related references: PMID:11156409 PMID:15073321 PMID:8087842
• Upon genotoxic stress, Caspase-2 associates with PIDD1 and CRADD to form the PIDDosome complex, which mediates its activation and triggers apoptosis.
• Subcellular localization includes the nucleus and cytoplasm, consistent with its roles in both nuclear and cytoplasmic apoptotic signaling.
• Tissue expression is highest in embryonic lung, liver, and kidney, and in adult placenta, lung, kidney, and pancreas, with lower levels in heart, brain, liver, and skeletal muscle.
• Post-translational modifications include acetylation and phosphorylation, and the protein is synthesized as an inactive zymogen requiring proteolytic processing for activation.
• Mutations in the CASP2 gene are associated with neurological disorders such as intellectual disability and lissencephaly, highlighting its non-apoptotic roles in neurodevelopment.
• Alternative splicing generates multiple isoforms, possibly contributing to its dual pro- and anti-apoptotic functions.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band around 51kDa corresponding to the full-length proenzyme; activation-dependent cleavage products may also be observed.
• The immunogen covers the C-terminal region (aa 334–452); epitope accessibility to both pro- and cleaved forms should be assessed in apoptosis-induced cell lysates.
• For ELISA, the recombinant protein used as immunogen can serve as a positive control or standard.
• Cross-reactivity has been confirmed with human samples; verification is recommended for other species due to potential sequence divergence.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies a wide range of IVD raw materials, including this Caspase-2 Rabbit mAb for apoptosis research. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support your assay development. For custom sourcing of rare targets or technical consultation, contact our team.