Anti-Caspase-14 Polyclonal Antibody for WB, ELISA - P31944

Core Product Specifications and Parameters

Biological Background: Caspase-14 Function and Localization

• Caspase-14 is a non-apoptotic caspase predominantly involved in epidermal differentiation and keratinocyte cornification. Related references: PMID:15556625
• It processes filaggrin, essential for skin barrier formation, and shows substrate preference for WEHD-ACF in vitro. Related references: PMID:16854378, PMID:19960512
• Involved in retinal pigment epithelium cell barrier function. Related references: PMID:25121097
• Participates in DNA degradation during keratinocyte differentiation by cleaving DFFA/ICAD, leading to DFFB/CAD liberation. Related references: PMID:24743736
• Subcellular localization includes both cytoplasm and nucleus.
• Tissue‑specific expression in skin suprabasal layers, hair shaft, and sebaceous glands; expression is very low in psoriatic skin. Related references: PMID:11175259, PMID:22825846
• Exists as a zymogen and undergoes proteolytic processing during terminal differentiation; the mature p17/10 form is found in the stratum corneum, while the intermediate p20/p8 form is in granular cells.

Experimental Guidance and Technical Tips

• The immunogen is a full‑length recombinant protein, so the polyclonal antibody may recognize multiple epitopes; include appropriate positive and negative controls in your assay.
• For WB, the predicted molecular weight is 28kDa; validate band size in your sample system to account for potential processing intermediates.
• Cross‑reactivity with mouse and rat has been demonstrated; titration experiments are recommended when switching species.
• Expected applications include WB and ELISA; for other platforms, perform a pilot validation under your experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with a one‑stop supply chain for IVD raw materials. This Caspase‑14 polyclonal antibody is suitable for dermatological and apoptosis research, and we can source validated antibody pairs, bulk ancillary reagents, or rare‑target raw materials to streamline your development pipeline.